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Table 3 Candidates genes ranked according to their expression stability estimated using geNorm algorithm after stepwise exclusion of the least stable reference gene

From: Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

Plant organs Flower buds Floral organs Fruits Total
Ranking Stability value (M) Ranking Stability value (M) Ranking Stability value (M) Ranking Stability value (M) Ranking Stability value (M)
GhACT4 0.558 GhACT4 0.491 GhFBX6 0.32 GhMZA 0.422 GhPP2A1 0.59
GhEF1α5 0.558 GhPP2A1 0.491 GhMZA 0.32 GhPTB 0.422 GhPTB 0.59
GhPP2A1 0.634 GhPTB 0.539 GhPTB 0.396 GhUBQ14 0.58 GhMZA 0.682
GhFBX6 0.686 GhβTUB3 0.578 GhPP2A1 0.433 GhPP2A1 0.628 GhUBQ14 0.747
GhUBQ14 0.768 GhUBQ14 0.604 GhβTUB3 0.519 GhACT4 0.785 GhACT4 0.777
GhMZA 0.824 GhEF1α5 0.644 GhACT4 0.595 GhβTUB3 0.901 GhEF1α5 0.825
GhPTB 0.859 GhFBX6 0.678 GhUBQ14 0.682 GhEF1α5 1.09 GhFBX6 0.85
GhGAPC2 0.959 GhMZA 0.752 GhEF1α5 0.739 GhFBX6 1.21 GhβTUB3 0.894
GhβTUB3 1.024 GhGAPC2 0.973 GhGAPC2 0.821 GhGAPC2 1.34 GhGAPC2 1.024
  1. Stability values are listed from the most stable genes to the least stable.