Table 3 Candidates genes ranked according to their expression stability estimated using geNorm algorithm after stepwise exclusion of the least stable reference gene
Plant organs | Flower buds | Floral organs | Fruits | Total | |||||
|---|---|---|---|---|---|---|---|---|---|
Ranking | Stability value (M) | Ranking | Stability value (M) | Ranking | Stability value (M) | Ranking | Stability value (M) | Ranking | Stability value (M) |
GhACT4 | 0.558 | GhACT4 | 0.491 | GhFBX6 | 0.32 | GhMZA | 0.422 | GhPP2A1 | 0.59 |
GhEF1α5 | 0.558 | GhPP2A1 | 0.491 | GhMZA | 0.32 | GhPTB | 0.422 | GhPTB | 0.59 |
GhPP2A1 | 0.634 | GhPTB | 0.539 | GhPTB | 0.396 | GhUBQ14 | 0.58 | GhMZA | 0.682 |
GhFBX6 | 0.686 | GhβTUB3 | 0.578 | GhPP2A1 | 0.433 | GhPP2A1 | 0.628 | GhUBQ14 | 0.747 |
GhUBQ14 | 0.768 | GhUBQ14 | 0.604 | GhβTUB3 | 0.519 | GhACT4 | 0.785 | GhACT4 | 0.777 |
GhMZA | 0.824 | GhEF1α5 | 0.644 | GhACT4 | 0.595 | GhβTUB3 | 0.901 | GhEF1α5 | 0.825 |
GhPTB | 0.859 | GhFBX6 | 0.678 | GhUBQ14 | 0.682 | GhEF1α5 | 1.09 | GhFBX6 | 0.85 |
GhGAPC2 | 0.959 | GhMZA | 0.752 | GhEF1α5 | 0.739 | GhFBX6 | 1.21 | GhβTUB3 | 0.894 |
GhβTUB3 | 1.024 | GhGAPC2 | 0.973 | GhGAPC2 | 0.821 | GhGAPC2 | 1.34 | GhGAPC2 | 1.024 |