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Table 4 Cotton reference genes for normalization and their expression stability values calculated by the NormFinder software

From: Identification and evaluation of new reference genes in Gossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

Plant organs Flower buds Floral organs Fruits Total
Ranking Stability value Ranking Stability value Ranking Stability value Ranking Stability value Ranking Stability value
GhPP2A1 0.24 GhACT4 0.233 GhFBX6 0.179 GhMZA 0.093 GhPP2A1 0.277
GhUBQ14 0.359 GhPP2A1 0.326 GhMZA 0.266 GhPTB 0.162 GhUBQ14 0.352
GhMZA 0.375 GhUBQ14 0.339 GhPTB 0.278 GhUBQ14 0.183 GhACT4 0.362
GhEF1α5 0.379 GhPTB 0.361 GhACT4 0.3 GhPP2A1 0.189 GhMZA 0.364
GhPTB 0.564 GhEF1α5 0.367 GhPP2A1 0.302 GhACT4 0.268 GhPTB 0.37
GhFBX6 0.578 GhβTUB3 0.368 GhβTUB3 0.352 GhGAPC2 0.506 GhEF1α5 0.445
GhACT4 0.595 GhFBX6 0.463 GhUBQ14 0.479 GhβTUB3 0.561 GhFBX6 0.464
GhGAPC2 0.657 GhMZA 0.532 GhEF1α5 0.503 GhEF1α5 0.591 GhβTUB3 0.481
GhβTUB3 0.721 GhGAPC2 0.969 GhGAPC2 0.58 GhFBX6 0.647 GhGAPC2 0.714
Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value Best combination Stability value
GhUBQ14 and GhPP2A1 0.180 GhACT4 and GhUBQ14 0.222 GhACT4 and GhFBX6 0.187 GhMZA and GhPTB 0.109 GhPP2A1 and GhUBQ14 0.221
  1. Stability values are listed from the most stable genes to the least stable.