Table 4 Cotton reference genes for normalization and their expression stability values calculated by the NormFinder software
Plant organs | Flower buds | Floral organs | Fruits | Total | |||||
|---|---|---|---|---|---|---|---|---|---|
Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Ranking | Stability value |
GhPP2A1 | 0.24 | GhACT4 | 0.233 | GhFBX6 | 0.179 | GhMZA | 0.093 | GhPP2A1 | 0.277 |
GhUBQ14 | 0.359 | GhPP2A1 | 0.326 | GhMZA | 0.266 | GhPTB | 0.162 | GhUBQ14 | 0.352 |
GhMZA | 0.375 | GhUBQ14 | 0.339 | GhPTB | 0.278 | GhUBQ14 | 0.183 | GhACT4 | 0.362 |
GhEF1α5 | 0.379 | GhPTB | 0.361 | GhACT4 | 0.3 | GhPP2A1 | 0.189 | GhMZA | 0.364 |
GhPTB | 0.564 | GhEF1α5 | 0.367 | GhPP2A1 | 0.302 | GhACT4 | 0.268 | GhPTB | 0.37 |
GhFBX6 | 0.578 | GhβTUB3 | 0.368 | GhβTUB3 | 0.352 | GhGAPC2 | 0.506 | GhEF1α5 | 0.445 |
GhACT4 | 0.595 | GhFBX6 | 0.463 | GhUBQ14 | 0.479 | GhβTUB3 | 0.561 | GhFBX6 | 0.464 |
GhGAPC2 | 0.657 | GhMZA | 0.532 | GhEF1α5 | 0.503 | GhEF1α5 | 0.591 | GhβTUB3 | 0.481 |
GhβTUB3 | 0.721 | GhGAPC2 | 0.969 | GhGAPC2 | 0.58 | GhFBX6 | 0.647 | GhGAPC2 | 0.714 |
Best combination | Stability value | Best combination | Stability value | Best combination | Stability value | Best combination | Stability value | Best combination | Stability value |
GhUBQ14 and GhPP2A1 0.180 | GhACT4 and GhUBQ14 0.222 | GhACT4 and GhFBX6 0.187 | GhMZA and GhPTB 0.109 | GhPP2A1 and GhUBQ14 0.221 | |||||