Figure 1

The gradient of [Ca²⁺] _m in root hairs was disrupted by Lat-B and Jas. (A1) Fluorescence micrograph of mitochondria (red particles) labeled with Rhod-2 in a normally growing root hair. (A2) Superimposition of the bright field image and fluorescence micrograph of the same root hair. Scale bars = 10 μm. (B) The gradient distribution of mitochondria in root hairs did not obviously change after a 10-min treatment with 500 nM Lat-B. The graph depicted the number of mitochondria from a 10-μm distance from the apex to the base region at 10-μm intervals. CK represented the measured data before treatment. (C) The gradient of [Ca²⁺]_m in a normal root hair was disrupted after a 3-min treatment with 500 nM Lat-B. The graph showed the concentration from a 10-μm distance from the apex to the base region at 10-μm intervals. CK represented the measured data in drug-free medium before treatment. (D) The gradient of [Ca²⁺]_m was dissipated after 3 min of treatment with Jas. CK represented the measured data in drug-free medium before treatment. (E) Decreases in [Ca²⁺]_m in the tip, shank, and base regions of the root hair after a 10-min treatment with 500 nM Lat-B. F0 represented the fluorescence intensity value of Rhod-2 before treatments, and F represented the fluorescence intensity value in same mitochondria after treatments.