Figure 2

Changes in [Ca²⁺] _m induced by Lat-B and Jas treatments. [Ca²⁺]_m was assessed based on fluorescence intensity of Rhod-2, as described in the Methods section. (A) and (C) The diamond curve showed the decrease in [Ca²⁺]_m induced by 500 nM actin drugs treatment with in 20 min; the triangle curve showed the DMSO vehicle control; the square curve represented the buffer control in drug-free medium; and the dotted curve showed the change in [Ca²⁺]_m with 30-min pretreatment with 2 μM Cs A before actin drugs application. (B) and (D) The effects of actin drugs on [Ca²⁺]_m at different concentrations. Root hairs were treated with 0 (DMSO), 10, 100, 250, 500 nM and 1 μM actin drugs for 10 min. The graphs showed the dose-dependent effect of actin drugs in the 10-500 nM concentration range on [Ca²⁺]_m in the root hair. These data were from concentration changes of mitochondria in sub-tip area of the root hair. F0 represented the fluorescence intensity value of Rhod-2 before treatments, and F represented the fluorescence intensity value in same mitochondria after treatments.