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Figure 3 | BMC Plant Biology

Figure 3

From: The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria

Figure 3

Phenotype of the AtYLMG1-1 knockdown plant. (A) Three-week-old seedlings of the wild type (WT) and the AtYLMG1-1 knockdown line (AS#1 and AS#2). Bars = 10 mm. (B) Levels of AtYLMG1-1 protein in the wild type and the two AtYLMG1-1 knockdown lines. Total proteins extracted from the wild type (WT) and the two AtYLMG1-1 knockdown lines (AS#1 and AS#2) were analyzed with the anti-AtYLMG1-1 antibodies. Fifty micrograms of proteins were loaded in each lane. The Rubisco small subunit (Rubisco SSU) was detected by CBB staining as the quantitative control. (C) Levels of AtYLMG1-1 and other YLMG gene transcripts in the wild type and two independent AtYLMG1-1 knockdown lines (AS#1 and AS#2). The levels of AtYLMG1-1, AtYLMG1-2, AtYLMG2, and AtYLMG3 transcripts were analyzed by RT-PCR in the wild type, AS#1, and AS#2. UBQ1 was used as the quantitative control. (D) Chloroplasts in leaf mesophyll cells of the wild type and the AtYLMG1-1 knockdown line. Chloroplasts in expanding leaf cells and the basal part of expanding leaf cells of the wild type (WT) and the AtYLMG1-1 knockdown line (AS#1) are shown. Bars = 10 μm. (E) Localization of FtsZ in the wild type and the AtYLMG1-1 knockdown line (AS#1). Localization of FtsZ2-1 in mesophyll cells was examined by immunofluorescence microscopy. The green fluorescence shows the localization of FtsZ2-1 and the autofluorescence of chlorophyll is depicted in red. Bars = 5 μm.

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