Effect of ERS1 overexpression on etr2 ein4 ers2. (A) adult phenotype of etr2 ein4 ers2, class A (L1, L2, and L3) and class B (S1, S2, and S3) lines carrying the 35S:ERS1 transgene. (B) immunoassay of ERS1 level in individual transformed lines. The ERS1 protein is not detectable in the ers1 mutant ers1-2 etr2 ein4. (C) relative CHIB gene expression in etr2 ein4 ers2 and transformed lines. (D) relative RTE1 gene expression in receptor mutants. Chemiluminance for the immunoassay was captured by a cold CCD (at -110°C and displayed in pseudo-color; the pseudo-color bar (CHEM) indicates relative signal strength from weak (dark) to strong (bright). ERS1: relative ERS1 accumulation in immunoassay probed with ERS1 antibody. Stained blot: membrane was stained with Coomassie Blue after the immunoassay to indicate relative protein amount. NA: data not available. Arrows indicate senesced leaves. Error bars indicate standard deviation. P: probability of a larger F value. n = 3 × 3: each measurement was repeated three times from three independent biological materials. Common mutations are highlighted in a box.