Expression levels of lycopene β-cyclase ( CYC-B ) in different tissues of S. lycopersicum and S. habrochaites. RT-PCR was performed with total RNA isolated from leaves, flowers and fruits at different stages of development. (A) Wild-type tomato cv. Pusa Ruby (S. lycopersicum); Lanes 2-3, flower and leaf, respectively; Lanes 4-7; fruit at different stages of ripening, from mature green (lane 4), breaker (Lane 5), orange (lane 6) to red ripe stage (Lane 7). (B) S. habrochaites genotype EC520061. Lanes 2-3, flower and leaf, respectively; Lanes 4-7, fruit with progressive stages of ripening, from mature green (Lane 4) to ripe stage (Lane 7). In case of S. habrochaites, which is a green fruited genotype, progress of ripening was broadly defined on the basis of seed color and development (see Materials and Methods). Lanes 1 and 8, 1 kb Mol. weight marker.