Cloning of ShCYC-B full-length promoter and its deletion fragments in binary vector. (A) PCR amplification of full-length and 5' deletion fragments of ShCYC-B promoter. M, 1 kb Mol. wt. marker; Lanes 1-4, amplicons of 908 bp, 818 bp, 578 bp and 436 bp, respectively. (B) Restriction confirmation of cloning of full-length and deletion fragments of ShCYC-B promoter in binary vectors. M, 1 kb Mol. wt. marker; Lanes 1-5, plasmids pBI121, pD0-908, pD1-818, pD2-578 and pD3-436, respectively, restricted with HindIII and BamHI. (C) Schematic illustrations of ShCYC-B promoter and its deletion fragments. The numbers on the left indicate the 5' end points of the promoter fragments relative to the translational start site. Binary vector pBI121 having GUS gene driven by CaMV35S promoter was used as a positive control.