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Figure 1 | BMC Plant Biology

Figure 1

From: SuperSAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): identification of early mediators of insect responses

Figure 1

Schematic representation of the approach used for identification of regulatory genes by SuperSAGE. Two SuperSAGE libraries were generated from the second fully expanded leaf of N. attenuata. Plants were mechanically wounded or wounded plus the immediate addition of 18:3-Glu as a single FAC elicitor and leaves were harvested 30 min after the treatments. From these libraries, 547 unique mRNA sequences (UniTags) were defined as differentially expressed after 18:3-Glu elicitation versus wounding (FC: fold-change). After gene ontology categorization, the kinetics of transcript accumulation corresponding to 27 UniTags were analyzed by quantitative PCR. Six selected genes were functionally characterized by Virus Induce Gene Silencing (VIGS).

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