Skip to main content

Table 5 Butte 86 gamma gliadins identified by MS/MS from wheat flour.

From: Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour

Butte 86 contig #

Band # 1

Total # peptides 2

% Coverage3

Unique peptides4

Enzyme used to generate peptide5

2

126

14

36.0

SQQQQVGQGSL

CH

    

QQLPQPQQPQQSFPQQQR

CH

    

SQQQQVGQGSLVQGQGIIQPQQPAQL

CH

    

NIQVDPSGQVQWLQQQLVPQLQQPL

CH

    

LQQPQQPFPQPQQQLPQPQQPQQ

TH

4

188

22

56.5

SIIMQQEQRQGVQIRRPL

CH

    

LQPQQPQQSFPQQQQPL

CH

    

VSPDCSTINAPF

CH

    

VSPDCSTINAPFASIVVGIGGQ

CH

    

LQPQQPQQSFPQQQQPLIQL

CH

    

LQPQQPQQSFPQQQQPLIQLSL

CH

    

CQQPQQTIPQPHQTF

CH

    

IIMQQEQRQG

TH

    

IIMQQEQRQGVQ

TH

    

LQPQQPQQSFPQQQQPLIQ

TH

    

VDPGYQVHWPQQQPFPQPQQP

TH

    

VHWPQQQPFPQPQQP

TH

    

NFLLQQCNPVSLVSSLISMILPR

TR

5

145

31

44.8

VPPECSIIRAPF6

CH

    

IQPSLQQR

CH

    

IQPSLQQRL

CH

    

SQQQQLGQGTL6

CH

    

QSFPQQQRPF

CH

    

ILLPLSQQQQL6

CH

    

TQQPQQPFPQFQQPHQPF6

CH

    

VQGQGIIQPQQLAQLEAIRSL6

CH

    

SQQQQLGQGTLVQGQGIIQPQQL6

CH

    

SQQQQLGQGTLVQGQGIIQPQQLAQLEAIRSL6

CH

    

ILLPLSQQQQLGQGTL6

CH

    

SQQQQLGQGTLVQGQGIIQPQQLAQL6

CH

    

SQQPQQAFPQPQQTFPHQPQQQVPQPQQPQQPF

CH

    

HQPQQQFPQPQQPQQSFPQQQRPF

CH

    

HQPQQQFPQPQQPQQSFPQ

CH

    

AFPQPQQTFPHQPQQQVPQPQQPQQPF

TH

    

FHQPQQQFPQPQQPQQ

TH

    

FHQPQQQFPQPQQPQQSFPQQQRP

TH

    

IQPSLQQR

TR

    

QLAQLEAIR

TR

    

PFIQPSLQQR

TR

    

QSFPQQQRPFIQPSLQQR

TR

6

186

16

36.5

RQPQQPF

CH

    

ASIVAGISGQ

CH

    

YQQPQQTFPQPQ

CH

    

LAQIPRQ

TH

    

IQILRPLFQ

TH

    

IIQPQQPAQYEVIRS

TH

    

FRQPQQPFY

TH

    

IIQPQQPAQYE

TH

    

YQQPQQTFPQPQQ

TH

    

FRQPQQPFYQQPQQTFPQPQQ

TH

    

FYQQPQQTFPQPQQ

TH

7

170

13

43.4

LQPHQPF

CH

    

ASIVASIGGQ

CH

    

QGVQILVPL

CH

    

SQQQQVGQGIL

CH

    

SQQQQVGQGILVQGQGIIQPQQPAQL

CH

    

VYVPPYCST

TH

    

LQPHQPFSQQPQQ

TH

    

APFASIVASIGGQ

TR

    

QPFPQQPQQPYPQQPQQPFPQTQQPQQPFPQSK

TR

11

149

4

27.6

YQQQQVGQGTLVQGQGIIQPQQPAQL

CH

    

SFPQQQPPF

TH

17

180

7

24.9

ASIVADIGGQ

CH

87

  

22.0

KAPFASIVADIGGQ

CH

    

APFASIVADIGGQ

TR

38

169

16

37.5

ANIDAGIGGQ

CH

108

  

39.7

GIIQPQQPAQLEGIRSLVL

CH

    

VPPNCSTINVPY

CH

    

VPPNCSTINVPYANIDAGIGGQ6

CH

    

VTILRPLFQ6

TH

    

INVPYANIDAGIGGQ

TH

    

NFLLQQCNHVSLVSSLVSIILPR

TR

  1. 1 detailed in Vensel et al. (in preparation).
  2. 2 obtained by interrogation of "subset" database with MS/MS data.
  3. 3 excluding 19 amino acid signal peptide.
  4. 4 sequences of peptides obtained by MS/MS that are not shared by other Butte 86 gamma gliadins. Peptide containing the extra cysteine is indicated in italics.
  5. 5 CH, chymotrypsin; TH, thermolysin; TR, trypsin.
  6. 6 peptides that distinguish Butte 86 sequence from closest NCBI match.
  7. 7 MS/MS data consistent with either Butte 86 gamma gliadin #1 or #8.
  8. 8 MS/MS data consistent with either Butte 86 gamma gliadin #3 or #10.