Tissue distribution of ProDH2 expression. A-C: ProDH2-promoter activity visualised in transgenic plants carrying a ProDH2-promoter-Gus fusion construct. Tissues were stained for 6 h in 1 mM X-Gluc and destained in 80% (v/v) EtOH. A: Two mature leaves of a 6-week-old plant. B: Three-week-old seedling showing enhanced staining with progressing leaf age. C: Inflorescence and young siliques showing GUS activity in the abscission zone between the pistils and the floral stalk. D: Northern blot analysis of transcript levels of Pro degradation genes in various tissues and two different cell cultures. The picture of the EtBr stained gel is shown to demonstrate RNA integrity and equal loading.