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Table 1 PCR primers used in this study

From: Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis

Name1

Sequence (5'->3')

Used for

Pdh1-LP1 (A)

tctcctctatcccaacctctg

pdh1-1, GABI_308F08 (pdh1-3), SALK_119334 (pdh1-4)

Pdh1-RP1 (B)

gatcgctcactcgtttcagaag

 

Pdh1-LP2 (C)

aagttggtgagaggggcttac

SALK_081276 (pdh1-2)

Pdh1-NS-r (D)

cgcaatcccggcgattaatctc

ProDH1 cloning

Pdh1-f

caccataATGgcaacccgtcttctc2

 

Pdh2-LP

gtaaccagcccctaaacctc

SALK_108179, GABI_918D08, SAIL_90_G05

Pdh2-RP

ggagtactagatcgcgtgtaac

 

Pdh2-LP2 (F)

aaaccctaccttcgtctcac

GT1788 (pdh2-1), GABI_328G05 (pdh2-2)

Pdh2-RP2 (G)

cactaacccgttttaggacattc

 

Pdh2-LP3

gagaagagttatggcttggtg

GABI_187C05

Pdh2-RP3

atgtccctttgtaatctgaattgg

 

Pdh2-f (E)

caccataATGgcaaaccgtttcctc2

ProDH2 cloning

Pdh2-NSr

ccaagccataactcttctcttaag

 

Pdh2-Prom-f

catttggatccttaccatccac

ProDH2 promoter cloning

Pdh2-Prom-r

cgggatccgtttgcCATttaaactc2

 

Salk_LBb2

ttcggaaccaccatcaaacag

SALK lines

Gabi-LB

cccatttggacgtgaatgtagacac

GABI lines

Sail-LB2

gcttcctattatatcttcccaaattaccaataca

SAIL_90_G05

Gus5'rev

atttcacgggttggggtttc

GT1788

Put1KO-f

aaacatcgctacatagtaataacactaacgcacgcta

gaaCGGATCCCCGGGTTAATTAA3

Put1 knockout

Put1KO-r

ttggtttgtctttgaaattggagtatatattatagtcctcG

AATTCGAGCTCGTTTAAAC3

 

SDH-f

caccacgagaataaagATGctatcgct2

SDH-MTP

SDH-Pdh1-r

cttgttgtccaaaggagagCTCGTGATCTATTATGTG4

SDH-MTP ProDH1 fusion

SDH-Pdh2-r

ggttggtcaaaggaaaggatCTCGTGATCTATTATGTG4

SDH-MTP ProDH2-fusion

Put1-f

caccctagaaATGatagcttcc2

Put1 cloning

Put1-r

taggcctactctttttggaatc

 

Pdh1-pr-r

atggtcataaaacgtacttttcac

northern probes

(with Pdh1-f or Pdh2-f)

Pdh2-pr-r

gactcatacacgctactcac

 

P5CS1-f

aatgagaggaaaaggacaag

P5CS1 probe

[8]

P5CS1-r

gataggatatgagtactaagcagag

 

P5CDH-f

tggacagaagtgttctgcac

P5CDH probe

P5CDH-r

gcttccaacactagaggaag

 
  1. 1 Letters in brackets refer to the abbreviations in Figure 6; 2 capital letters indicate the translation start codon; 3 capital letters indicate the nucleotides specific for the resistance cassette, lower case letters correspond to Put1 5' and 3' sequences; 4 capital letters correspond to the 3' sequence of the SDH-MTP, lower case letters correspond to the nucleotides encoding the N-terminus of the predicted mature Arabidopsis ProDH proteins.
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BMC Plant Biology

ISSN: 1471-2229

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