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Figure 6 | BMC Plant Biology

Figure 6

From: Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)

Figure 6

Relative quantification of two LuMYB1 splice forms. The relative expression levels of LuMYB1-1 (A, B, C and D) and LuMYB1-2 (E, F, G and H) were determined after normalization. The total tissue (A and E) or stem (C and G) sample sets were normalized by geNorm with EF1A, ETIF5A and UBI or with ETIF1 and ETIF4F respectively. The NormFinder algorithm was also used with different combinations of HKG. The total tissues (B and F) were divided in 1, 2 or 3 groups and normalized with GAPDH alone (1 group, yellow bar), EF1A and EF2 (stem/others, pink bar), EF1A and ETIF5A (apical/medium/others, green bar) ETIF3E and UBI (external/internal/others, blue bar). When the expression was measured only in one group containing the stem tissues (D and H), ETIF3H was used for normalization. V: vegetative F: flowering and C: green capsule stages; A: apical and B: basal parts of the stem; I: internal and E: external stem tissues; LEA: leaves; FLO: flowers. Values = relative expression levels +/- SD.

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BMC Plant Biology

ISSN: 1471-2229

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