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Figure 4 | BMC Plant Biology

Figure 4

From: Identification and characterization of NAGNAG alternative splicing in the moss Physcomitrella patens

Figure 4

Example of the validation procedures employed. A: Electropherogram of the direct sequencing of a cDNA PCR product. Starting with the NAGNAG AS site at position 132 the two alternatively spliced sequence signatures are overlaid. B: FAM fluorescence intensity peaks of the two splice variants (length difference three nucleotides). The lower peak constitutes 40% of the area (ar) of the bigger one, i.e. an approximate ratio of 3:1.

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