Figure 6
Molecular interaction between ZmTCRRs and ZmHPs. a, growth of AH109 yeast cells carrying ZmTCRRs-binding domain and ZmHPs-activation domain fusions on histidine-lacking medium. Upper left image, growth control on non selective medium after 10 days. Upper right image, distribution of ZmHPs-activation domain clones on the interaction plates. Lower left image, interaction plate for ZmTCRR-1 after 5 days; lower right image, interaction plate for ZmTCRR-2 after 10 days. b, quantification of interaction based on galactosidase activity in a 2-hybrid assay in YRG-2 cells. White bars, interaction of the ZmTCRR-1-activation domain fusion protein with ZmHP-binding domain fusions. Grey bars, interaction of the ZmTCRR-2-activation domain peptide with ZmHP-binding domain fusions. A yeast clone carrying only ZmTCRR-1-activation domain was used as to establish the background level. Each bar represents the mean and standard deviation of 3 independent experiments.