Table 2 Sequences of the PCR primers used in this work.
CLONING OF CODING SEQUENCES | |
|---|---|
TCRR2-GWS | AAAAAGCAGGCTCCATGGACACTATTGGTCCAC |
TCRR2-GWAS | AGAAAGCTGGGTATCAAATATAGCTCAAGATACGAGG |
HP1.3-GWS | AAAAAGCAGGCTCCATGTCTGCCGCGAACCAGC |
HP1-GWAS | AGAAAGCTGGGTTACTTGTTGGGGGGAAAGC |
HP3-GWAS | AGAAAGCTGGGTCACTTGCTGGGGGGAC |
HP2-GWS | AAAAAGCAGGCTCCATGGCTGCCGCCGCGC |
HP2-GWAS | AGAAAGCTGGGTTATTGTTGAGCCTGGATTTGC |
AttB recombination motifs are shown underlined | |
CLONING OF ZmTCRR-1 AND ZmTCRR-2 PROMOTER | |
TCRR1p-GWS | AAAAAGCAGGCTAGCTTCATAGGATGATCCAC |
TCRR1p-GWAS | AGAAAGCTGGGTGGACTAGCTAGACAAGCTC |
TCRR2p-GWS | AAAAAGCAGGCTTAGTGTGCAATCGAAGCAACGG |
TCRR2p-GWAS | AGAAAGCTGGGTAGATACTCTCCCACAACTTCC |
AttB recombination motifs are shown underlined | |
qRT-PCR PRIMERS | |
HP1.3-QPCRS | AACACTTGCATTCAGTTCCGCG |
HP1-QPCRAS | CCAGTACCTTGAGGCACCCATCTC |
HP3-QPCRAS | CCAGTGTCTTGAGGCACCCATCTT |
HP2-QPCRS | TCGTCACCCTCTTCTGCGACG |
HP2-QPCRAS | CCACGATGGGCTGGTCAAGC |
TCRR2-QPCRS | ATTCAAGTGACAATGGTGGAGGG |
TCRR2-QPCRAS | CCAGGCATACAATAATCGGTCAGA |
FKBP-QPCRS | GGGTGCTGTTGTTGAAGTCA |
FKBP-QPCRAS | GCAATAACTTCCTCTTCATCG |
HK1-QPCRS | GTGTGGCAGAGCATTGATTACAC |
HK1-QPCRAS | TCACATACAAATACGGCAAGCTCA |
HK1a2-QPCRS | GTGTGGCAGAGCATTGATAACGT |
HK1a2-QPCRAS | ACTGCAAGCTCAATGCACTTCTCC |
HK1b1-QPCRS | AATGGCAGTTCTCTAACCAGCACG |
HK1b1-QPCRAS | TTTTGGGCAATCCAGGTGGACC |
HK1b2-QPCRS | CGCTAATCAATGAAGTGCTTGACAG |
HK1b2-QPCRAS | GATTCAAGATCCAACTTTCTGGC |
HK2-QPCRS | CACAGGAGAAAGGACTGGAGTTGG |
HK2-QPCRAS | GGATCGCCAATTAGTGTTTGTGG |
HK3A-QPCRS | GTCATGCACCTGCAGTATTGG |
HK3A-QPCRAS | TACATAAGTCACATTCATGCGGATT |
HK3B-QPCRS | GCGATCGGCAGCATATTTGGAA |
HK3B-QPCRAS | GCTGCGGAAACCAGACCAAAC |
C4pepQPCR-S | GAGGCTCTGCAGAGAGAGATCC |
C4pepQPCR-AS | CCATAGCGCATTTCGGCCTG |
ZmRR2QPCR-S | GCGCAGCTCCAAGTACAGAGTGAC |
ZmRR2QPCR-AS | TGTTCACGTCGGGGACCAGC |