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Table 1 Identified mutations in the AsPAL1 and AsCslF6 genes.

From: Development and characterization of an oat TILLING-population and identification of mutations in lignin and β-glucan biosynthesis genes

*Gene

Type

Amino acid

Position

PSSM Shift

SIFT

Method

AsPAL1

C to T

Thr to Ile

487

-6

Not Tolerated

MALDI-TOF and sequencing

AsPAL1

C to T

Thr to Met

532

0

Not Tolerated

MALDI-TOF and sequencing

AsPAL1

C to T

Pro to Ser

537

-

Tolerated

MALDI-TOF and sequencing

AsPAL1

C to T

Silent

 

-

-

MALDI-TOF and sequencing

AsPAL1

G to A

Silent

 

-

-

MALDI-TOF

AsPAL1

G to A

Silent

 

-

-

MALDI-TOF

AsCslF6

G to A

Val to Met

 

0

Tolerated

Sequencing

AsCslF6

G to A

Gly to Arg

 

-3

Tolerated

Sequencing

AsCslF6

C to T

Silent

 

-

-

Sequencing

AsCslF6

G to A

Silent

 

-

-

Sequencing

AsCslF6

G to A

Silent

 

-

-

Sequencing

AsCslF6

C to T

Silent

 

-

-

MALDI-TOF

AsCslF6

A to T

Met to Lys

 

-3

Tolerated

MALDI-TOF

AsCslF6

G to A

Silent

 

-

-

MALDI-TOF

AsCslF6

G to A

Gly to Asp

 

-2

Tolerated

MALDI-TOF

AsCslF6

G to A

Arg to His

 

-1

Tolerated

MALDI-TOF

  1. *Gene: The genes screened for; Type: detected mutation; Amino acid: the predicted amino acid change in the protein; Position: Position of the changed amino acid in the protein; PSSM and SIFT: significance of change in the amino acids; Method: "MALDI-TOF and sequencing" indicates that the same mutation was identified by both MALDI-TOF and DNA sequencing. Other mutants were either identified by MALDI-TOF or by DNA sequencing, as indicated.