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Figure 1 | BMC Plant Biology

Figure 1

From: The role of actin isoforms in somatic embryogenesis in Norway spruce

Figure 1

Phenotype and viability of Norway spruce somatic embryos during maturation on media containing latrunculin B. Vital staining of ESM with trypan blue (A, B) or with fluorescein diacetate (D, E, G, H, I-L). (A, B) ESM maturing for one week on a control medium (A) or a medium supplemented with 50 nM Lat B (B). Suspensors in controls consist of long viable cells (A), whereas most suspensor cells are dead in embryos maturing in the presence of Lat B (B). (C-H) ESM maturing for two weeks on a control medium (C-E) or a medium containing 50 nM Lat B (F-H). Long viable cells form long suspensors in the controls (D, E), whereas suspensors in the presence of Lat B consist of few short cells (G, H). Fluorescein diacetate staining revealed numerous granules in the cytoplasm of these short cells (arrows in H), indicating dying cells. (I-L) Three weeks of maturation. Cotyledons have developed in both treatments (I, K). A high degree of suspensor cell degradation is visible in the controls (J) and only few suspensor cells remain in embryos maturing in the presence of 50 nM Lat B (L). Scale bars, 200 μm.

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