Figure 2

Effect of latrunculin B on the yield and quality of somatic embryos. (A, B) ESM after four weeks of maturation. Numerous embryos at various stages of development formed in the controls (A), whereas fewer uniform embryos at advanced developmental stage formed in the presence of 100 nM Lat B (B). (C) Comparison between the number of embryos and their length after four weeks of maturation in the controls or on media containing various Lat B concentrations. Each point in the graph corresponds to one embryo and all embryos in each variant were arranged descending by size. The table shows percentage representation of embryos longer and shorter than 2.5 mm from the experiment shown in the graph (see Methods for details on the yield evaluation). The embryo length was established as a simple criterion of embryo maturity (see inset), where the embryo length 2.5 mm was considered as the minimal length for matured embryos. (D) Germination of somatic embryos for 2 weeks. Embryos that had matured in the presence of Lat B had a high ability to germinate. Scale bars are 2 mm (A, B) or 5 mm (D).