Skip to main content
Figure 5 | BMC Plant Biology

Figure 5

From: The leader intron of AtMHX can elicit, in the absence of splicing, low-level intron-mediated enhancement that depends on the internal intron sequence

Figure 5

Determination of splicing efficiency. A. The primers used for RT-PCR are indicated on the illustration of the WT construct. The forward and reverse primers were derived from the first exon of the 5'UTR and from the GUS coding sequence, respectively. B. The results of the RT-PCR analysis. The analysis was not quantitative but qualitative (that is, the analysis shows the size of each transcript, but band intensity do not accurately reflect the relative abundance of the different transcripts). All the templates were cDNA except pWT, which was the plasmid that included the WT construct. The product of pWT thus indicated the size of unspliced WT transcript. The boxes separated by a long or short line indicate the expected size of unspliced transcripts including either the full or deleted LI, respectively. The two adjacent boxes indicate the size of correctly spliced transcripts.

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us