Figure 1
Transcriptional regulation of the CRKs in response to O 3 . Expression of all members of the CRK group of plant RLKs was analyzed by quantitative real-time RT-PCR (qPCR) in Col-0 plants exposed to 250 ppb O3 for 6 h. Samples were harvested at 1 or 8 h (6 h followed by 2 h recovery under clean air conditions) after the onset of the O3 treatment. Transcript levels were calculated by comparison of O3-exposed plants with corresponding control plants grown under clean air conditions harvested in parallel with the O3-treated plants. An expression level of one indicates no change in expression, increased expression is indicated by values larger than one while decreased expression is shown by values smaller than one. Increase in expression by 2-fold or higher is high-lighted in red and decrease in expression by 2-fold or more in green. NR - no reproducible data could be obtained for this gene. The experiment was repeated four times; fold change was calculated from the average normalized cycle difference of all biological repeats. Statistical significance (Benjamini-Hochberg FDR corrected p-value ≤ 0.1) is indicated with asterisks (see additional file 1).