Figure 7

Analysis of 35S:MtCBF4 transgenic Arabidopsis lines. (a) RT-PCR analysis of Arabidopsis 35S:MtCBF4 transgenic lines (L17 and L24) and the wild type (WT). (b) Comparison of plants subjected to 16 d drought stress treatment and control plants. (c) Percentage survival of L17 and L24 plants exposed to 16 d drought stress. Mean survival and standard deviation (SD) were calculated from the results of three replicated experiments each using more than 60 seedlings. Asterisk indicates that these plants had significantly higher survival rates under drought treatment than wild-type plants (Student's t-test, *P < 0.05). (d) Germination of L17 and L24 seeds in the presence of 220 mM NaCl. Mean germination and SD were calculated from the results of three replicated experiments each using more than 60 seeds. Asterisk indicates that these plants had significantly higher germination rates under 220 mM NaCl than wild-type plants (Student's t-test, **P < 0.01, *P < 0.05). (e) Root growth of L17 and L24 seedlings in the presence of different concentrations of NaCl. Three days after germination on MS agar plates, WT and transgenic seedlings were transferred to a new MS agar plate containing different concentrations of NaCl for 7 days. The seedling root lengths were measured with Image software. (f) Root lengths of the means of three replicated experiments. Error bars indicate SD (n = 18). Bars = 0.5 cm.