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Figure 3 | BMC Plant Biology

Figure 3

From: Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae

Figure 3

Analyses of AtPP2 gene expression. (a-d) Plants were treated with HrpNEa and sampled at 0 hpt (immediately after treatment) and 24 hpt. Gene expression was determined by Reverse transcriptase-polymerase chain reaction (RT-PCR) using EF1α as a reference gene, by real-time RT-PCR using EF1α and ACTIN2 genes as references, or by northern blot hybridization with specific probes. (a) RT-PCR analyses of gene expression in WT plants. AtPP2-A1 through AtPP2-A15 and AtPP2-B1 through AtPP2-B15 are abbreviated as A1 through A15 and B1 through B15, respectively. (b) Real-time RT-PCR analysis of AtPP2-A1 and AtPP2-A14 expression in WT plants. Gene transcript was quantified as mean ± SD (n = 4 repeats) relative to reference genes and normalized to null-template controls. (c) RT-PCR analyses to determine effects of the WT plant and AtPP2-A-modified mutants on expression of selected AtPP2-A genes. The sequence-indexed T-DNA insertion mutants are shown as ellipsis of prefixal atpp2-. (d) Northern blots hybridized with probes specific to AtPP2-A1 or EF1α. Both mutants are shown in abbreviated form.

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