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Figure 4 | BMC Plant Biology

Figure 4

From: A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity

Figure 4

VvMYB5bL loses its ability to physically interact with the bHLH transcription factor VvMYC1 in yeast. Yeast two-hybrid experiments have been performed by co-transformation with VvMYB5bR or VvMYB5bL proteins fused to GAL4 Activation Domain, and VvMYC1 fused to GAL4 DNA Binding Domain. Transformed yeasts were selected on SD-Leu-Trp- medium and tested for LacZ activation. β-Galactosidase activity results are the mean of three measurements of three independent yeast clones. Negative two-hybrid control refers to the control provided by the manufacturer. Error bars indicate SD.

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BMC Plant Biology

ISSN: 1471-2229

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