Figure 5

Analysis of VvMYB5b^R and VvMYB5b^L ectopic expression effect in tobacco plants flowers. (A) Flowers of VvMYB5b^R overexpressing plants showed an intense red coloration of petals and stamens, compared to control and VvMYB5b^L transgenic flowers. (B) Real time quantitative RT-PCR analysis of NtCHS (chalcone synthase), NtDFR (dihydroflavonol reductase) and NtANS (anthocyanidin synthase) transcript abundance in stamens and corollas. Gene expression is shown relative to NtUbiquitin transcript levels in each sample. Results are presented for three independent transgenic lines overexpressing either VvMYB5b^R or VvMYB5b^L, and compared to control plants. VvMYB5b indicate transgene transcript levels. Each bar represents the mean ±SD of three replicates (* P < 0.05 vs. control plants according to the ANOVA).