Figure 3

The Arabidopsis ACR11- and ACR12-GFP fusion proteins are localized to the chloroplast. Arabidopsis mesophyll protoplasts were transformed with ACR11- and ACR12-GFP constructs, which encode the full-length ACR11 protein, and the first 94 amino acids of ACR12 fused to GFP, respectively. Chloroplasts were visualized by red chlorophyll autofluorescence. The green fluorescent signals of ACR11- and ACR12-GFP colocalized with the red fluorescent signals of chlorophyll (merge). Arabidopsis protoplasts transformed with the empty vector are shown as controls for the subcellular localization of GFP. Scale bars are 10 μm.