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Figure 8 | BMC Plant Biology

Figure 8

From: Transcriptional analysis of cell growth and morphogenesis in the unicellular green alga Micrasterias(Streptophyta), with emphasis on the role of expansin

Figure 8

Phenotypes of Micrasterias denticulata cells transiently overexpressing MdEXP2-GFP observed by confocal fluorescence microscopy. Merged transmission light and GFP fluorescence single optical sections (B-I) or projection (A). Initial semicells not formed under MdEXP2-GFP overexpression marked by asterisk. (A) Undivided MdEXP2-GFP overexpressing cell. (B-I) Phenotypes of M. denticulata cells transiently overexpressing MdEXP2-GFP arranged according to phenotype severity. (B) Cell line 11. Upper semicell formed after the first cell division, exhibiting stimulated lobe elongation. The lobes are stretched and rounded instead of flattened at their tips. (C-E) Elongation growth is reduced, lateral lobes are fused. (C) Cell line 6. Lower semicell formed after the second, upper semicell after the third cell division. (D) Cell line 7. Lower semicell formed after the first, upper semicell after the second cell division. (E) Cell line 18. Upper semicell resulting from the first cell division, after which the cell died. (F-I) Cell line 13. Loss of growth polarity and planarity upon cell division. (G, H) Other focal sections of (F) showing that there are three growth planes instead of one. (I) Semicells fused upon the second cell division. Scale bar = 50 μm.

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