Figure 1

A series of deletions in the 5' regulatory region of the TRY gene. A) Schematic overview showing the relative positions of TRY promoter fragments with respect to the start codon of the different fragments. Each fragment is fused to the CaMV 35S minimal promoter and either to the GUS coding region or to the TRY CDS followed by the nopaline synthase terminator. Single fragments (A or B) or their fusion were used (A+B). B) Summary of the GUS expression data. We distinguish between the ubiquitous expression called "basal expression" and expression in trichomes. We found two categories, basal and trichome expression and expression only in trichomes. The percentage of analyzed independent T2 lines showing the respective expression category is provided. Data marked with a "*" showed exclusively weak staining as exemplified in Figure 2J. C) Overview of the rescue efficiency. It was determined by the ability to reduce trichome cluster formation in the try-JC mutant. The percentage of clusters relative to the number of trichome initiation sites was calculated on the first four leaves. Statistical difference for each rescue experiment in comparison to Col wild type or to the try mutant is determined through Student's t-test. The difference between the respective two means is significant for P < 0,01.