Figure 1
From: High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes
Design and testing of primers for first round PCR. a) Electrophoretic profile of the PCR products obtained from Langdon (1), Langdon 2D(2A) (2), Langdon 2D(2B) (3) by using homoeoallele specific primer pairs. b) Graphical representation of the first round PCR amplicons. For SBEIIa-A the selected regions are: fragment from exon II to V (A(II-V) ); from exon VI to IX (A(VI-IX)); from exon X to XIII (A(X-XIII)). For SBEIIa-B: from exon IV to IX (B(IV-IX)). For SBEIIa-D: from exon II to VI (D(II-VI)); from exon X to XIII (D(X-XIII) ). Red, green and blue arrows represent PCR primers specific for genome A, B and D, respectively.