Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana

BMC Plant Biology

Table 2 Mutations induced by C-ion irradiation

LET (keV μm^-1)	Dose (Gy)	Allele	Mutated gene	Type of mutation*	Size** (bp)	Position
22.5	250	C-27-gl1	GL1	BS	G→A	Chr.5: 10,363,437
		C-45-hy1	HY4	Del	2	Chr.4: 5,724,273-74
		C-48-amp1	AMP1	BS	A→T	Chr.3: 20,255,432
		C-55-hy1	HY4	Del	3	Chr.4: 5,725,528-30
		C-142-hy1	HY3	Del	1	Chr.2: 8,141,902
		C-162-gl1	TTG1	Del	51	Chr.5: 8,371,718-68
	450	C(450)-100-gl1	TTG1	Del	1	Chr.5: 8,372,723
		C(450)-124-hy1	HY3	BS	G→T	Chr.2: 8,143,452
		C(450)-135-hy1	HY1	Del	36	Chr.2: 11,342,207-42
		C(450)-139-pid1	PID1	RTL		See Figure 3
		C(450)-150-pid1	PID1	Ins	1	Chr.2: 14,590,504
		C(450)-154-hy1	HY4	Del	32,335	Chr.4: 5,697,598-730,031
30.0	400	C30-8-gl1		NM
		C30-9-gl2	GL2	Del	1	Chr.1: 30,038,621
		C30-39-hy1	HY2	Del	5	Chr.3: 2,805,174-78
		C30-73-gl1	TTG1	CR		See Figure 3
		C30-74-hy1	HY4	Del	2	Chr.4: 5,725,263-64
		C30-106-gl1	GL2	Del	1	Chr.1: 30,039,697
		C30-108-hy1	HY4	RTL		See Figure 3
		C30-148-amp1	AMP1	Del	1	Chr.3: 20,257,195
		C30-155-hy1	HY2	Del	4	Chr.3: 2,804,929-32
		C30-252-gl1	GL2	BS	C→A	Chr.1: 30,040,486
		C30-273-var1	VAR2	Del	23	Chr.2: 13,175,250-72

* BS, Base substitution; CR, complex rearrangement; Del, deletion; Ins, insertion; RTL, reciprocal translocation; TL, translocation, NM, no mutation in the genes sequenced in this study.
**For base substitutions, changed bases are specified.

ISSN: 1471-2229