Table 7 Primer sequences of selected putative avocado defence-related genes from Phytophthora cinnamomi infected avocado roots.
Sequence ID | Fwd primer (5'-3') | Rev primer (5'-3') | Product length (bp) | Annealing °C |
|---|---|---|---|---|
Thaumatin | CACCCTGTAGTTCACTCC | CCAGATGCTTACAGTTACC | 75 | 58.5 |
LRR resistance PLP | GACATTCTTATAGCCATC | ATAAACAATCTGATTTTG | 135 | 56 |
Metallothionein like protein type 2 | AGTCTTCATCCCTAATACATATCCC | GTTTGTGCGTGTCTGGTTTC | 76 | 58.5 |
Thaumatin-like protein | AAGCAGTCCTCAAGGTTC | TTTCCGTTAGTGTCAAAGC | 79 | 65 |
MLO protein | TCGTGGATGGAAGGAGTG | ATGGGCAAATCTAAATCTTGTTG | 85 | 58.5 |
Pathogenesis-related protein PsemI | GAAGATGGAGTACAAATAC | CACCTTGATGTGATAAAC | 82 | 58.5 |
Proflin-like protein | TTCGGTATCTATGATGAG | ACGATATGACATTCAATAG | 110 | 58.5 |
Putative universal stress protein | GACATTCTTATAGCCATC | ATAAACAATCTGATTTTG | 135 | 56 |
Cytochrome P450 like TBP | GTCAAAGTGAAGAAATTC | AATCTCGTTAATCCATTC | 119 | 58.5 |
18S | GTCAAAGTGAAGAAATTC | AATCTCGTTAATCCATTC | 59 | |
Actin | GAATCTGGACCATCTATTG | TACCAACCAAACCAAATC | 114 | 58.5 |