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Table 1 Verification of gene expression by qRT-PCR

From: Transcriptome analysis of ectopic chloroplast development in green curd cauliflower (Brassica oleracea L. var. botrytis)

Genes

RPKM white

RPKM green

Ratio green/white

qRT-PCR Ratio* green/white

Toc159

0

23.1

23.1

4.52

ACP

0

18.7

18.7

1.47

FtsH8

0

12.8

12.8

1.55

LHCB1.5

50.6

320

6.32

7.84

G4

7.8

45.5

5.83

2.7

FAR1

2

8.2

4.1

1.64

CRY2

4.4

18.6

4.23

6.91

HY5

0

7.1

7.1

4.09

PHOT2

2

6.5

3.25

2.06

LHCB5

24.4

90.1

3.69

4.02

GUN5

3.6

14.5

4.03

4.81

CIP1

14.7

4.1

0.28

0.27

LSH7

9.1

1.8

0.2

0.63

HSP70-1

59.2

0.5

0.01

0.0003

  1. *qRT-PCR was carried out with two biological repeats and three technical trials.