- Research article
- Open Access
The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis
BMC Plant Biology volume 11, Article number: 19 (2011)
Sulfate and phosphate are both vital macronutrients required for plant growth and development. Despite evidence for interaction between sulfate and phosphate homeostasis, no transcriptional factor has yet been identified in higher plants that affects, at the gene expression and physiological levels, the response to both elements. This work was aimed at examining whether PHR1, a transcription factor previously shown to participate in the regulation of genes involved in phosphate homeostasis, also contributed to the regulation and activity of genes involved in sulfate inter-organ transport.
Among the genes implicated in sulfate transport in Arabidopsis thaliana, SULTR1;3 and SULTR3;4 showed up-regulation of transcripts in plants grown under phosphate-deficient conditions. The promoter of SULTR1;3 contains a motif that is potentially recognizable by PHR1. Using the phr1 mutant, we showed that SULTR1;3 up-regulation following phosphate deficiency was dependent on PHR1. Furthermore, transcript up-regulation was found in phosphate-deficient shoots of the phr1 mutant for SULTR2;1 and SULTR3;4, indicating that PHR1 played both a positive and negative role on the expression of genes encoding sulfate transporters. Importantly, both phr1 and sultr1;3 mutants displayed a reduction in their sulfate shoot-to-root transfer capacity compared to wild-type plants under phosphate-deficient conditions.
This study reveals that PHR1 plays an important role in sulfate inter-organ transport, in particular on the regulation of the SULTR1;3 gene and its impact on shoot-to-root sulfate transport in phosphate-deficient plants. PHR1 thus contributes to the homeostasis of both sulfate and phosphate in plants under phosphate deficiency. Such a function is also conserved in Chlamydomonas reinhardtii via the PHR1 ortholog PSR1.
Sulfur and phosphorus are two of the most important macro-elements for plant growth. Given their vital roles in sustaining growth, and their participation in related metabolic pathways, plants have evolved coordinated and tightly controlled mechanisms to maintain intracellular sulfur and phosphorus homeostasis in response to varying levels of external element availability. One example of their interdependency is the rapid replacement of sulfolipids by phospholipids under sulfur deficiency, and the replacement of phospholipids by sulfolipids during phosphorus deficiency [1–4]. The responses of plants to phosphorus and sulfur deficiency have largely been examined considering each element separately; however, the interaction and crosstalk between sulfur and phosphorus signaling pathways has been poorly studied [5, 6].
In plants, sulfur is acquired from the soil in its inorganic form of sulfate by the root system [7, 8]. A major portion of the absorbed sulfate is transported into the vacuole and the remaining portion is loaded into the xylem and then transferred to the shoots . In leaves, sulfate is reduced in the chloroplast and then assimilated into organic sulfur compounds, such as methionine, cysteine and glutathione. Transport of sulfate is mediated by members of the SULTR gene family containing 12 members in Arabidopsis thaliana that are subdivided into four groups. Members of group 1 encode high-affinity sulfate transporters, such as SULTR1;1 and SULTR1;2, that are involved in sulfate uptake into the root [10, 11]. Sulfate limitation also involves redistribution of sulfate from source to sink organs through the phloem vessels, a process mediated by the phloem-localized high-affinity sulfate transporter SULTR1;3 . Group 2 encode low-affinity sulfate transporters and includes SULTR2;1, which is expressed in the xylem parenchyma and pericycle cells of roots and strongly up-regulated by sulfate deficiency . Group 3 is the largest group of sulfate transporters with five members. SULTR3;5 functions in synergy with SULTR2;1 in mediating low-affinity sulfate transport when expressed in yeast and participates in root-to-shoot sulfate transport . A role for several SULTR3 members, including SULTR3;4, in sulfate translocation within developing seeds has also been recently described . Group 4 contains only two members. SULTR4;1 and SUTR4;2 are localized to the tonoplast and are proposed to play a role in the efflux of sulfate into the cytoplasm . Seeds of the sultr4;1 mutant have an enhanced sulfate content . The role of the two members of the SULTR5 gene family in sulfate metabolism is uncertain, as SULTR5;2 has only been demonstrated to encode a high-affinity molybdate transporter .
At present, relatively little is known about transcription factors that participate in the control of sulfate transporters under sulfate deficiency [19, 20]. In Arabidopsis, only one gene encoding the transcription factor Sulfur Limitation 1 (SLIM1) has been shown to play a role in the regulation of the expression of several sulfate transporters, such as SULTR1;1, SULTR1;2 and SULTR4;2 . Sulfate limitation also induces the expression of microRNA miR395 in a SLIM1-dependent manner [22, 23]. In turn, mirR395 regulates the accumulation and allocation of sulfate through the targeting of members of the ATP sulfurylase gene family (APS1, APS3 and APS4) and the SULTR2;1 gene .
Some transcription factors participating in the response of plant to inorganic phosphate (Pi) deficiency have been identified, including PHR1 , WRKY75 , ZAT6  and MYB62 . The PHR1 transcription factor is viewed as a positive regulator of Pi starvation responses and is involved in the up-regulation of the IPS1 gene in Pi-deficient plants . In the promoter of IPS1, PHR1 binds the P1BS cis-acting element, defined as the imperfect palindromic sequence GNATATNC . Similar palindromic sequences have been identified in the promoter region of several genes induced by Pi deficiency and positively regulated by PHR1, including the Pi transporter PHT1;1, PHO1;H1 involved in root-to-shoot Pi transfer, and the SQD1 and DGD2 genes involved in sulfolipid and galactolipid biosynthesis, respectively [29, 30]. The phr1 mutant shows impairment in a broad range of Pi-deficiency responses, including decreased accumulation of anthocyanin, starch and sugars, altered Pi allocation between root and shoot, and decreased response of Pi starvation-induced genes [25, 31]. PHR1 has also been shown to influence the expression of microRNA miR399, and forms, along with PHO2, an important branch in the long-distance Pi signaling pathway [25, 29, 32–35]. While PHR1 expression is not regulated by Pi status , the protein is sumoylated by the SUMO E3 ligase SIZ1, revealing a possible post-translational mechanism for PHR1 regulation . Several microarray studies revealed that the promoter region of genes up-regulated by Pi deficiency, including genes systemically controlled by low Pi, are particularly enriched for the P1BS element relative to non-induced genes [37–40]. Analysis of gene expression profile and phenotypes of the phr1 mutant and a phr1 phr1-like (phl1) double mutant, combined with overexpression of PHR1, revealed that PHR1 and PHL1 act as central integrators of the Pi starvation response in Arabidopsis .
Fine tuning of the crosstalk between the regulation of phosphorus and sulfur homeostasis, both at the transcriptional and metabolic level, has been demonstrated in the unicellular alga Chlamydomonas reinhardtii  and in Saccharomyces cerevisiae [42, 43]. However, in higher plants, although evidence suggests a similar coordination between phosphorus and sulfur homeostasis, the molecular mechanisms that regulate the sulfate homeostasis in response to Pi availability remain largely unknown. Using bioinformatics analysis, we found that the P1BS cis-acting element was present in the promoters of the genes SULTR1;3 and SULTR2;1, raising the possibility of the involvement of PHR1 in the crosstalk between sulfate and Pi signaling pathways in Arabidopsis. We thus first studied the transcriptional regulation of these two genes, as well as of SULTR3;5 and SULTR3;4, in wild-type (WT) Arabidopsis and phr1 mutant grown on Pi-depleted medium. Our results showed that SULTR genes were differentially regulated at the transcriptional level by the Pi status in plants and that PHR1 could play a positive role in the expression of SULTR1;3, and a negative role in the expression of SULTR2;1 and SULTR3;4. Under Pi-deficient conditions, the sulfate shoot-to-root transfer capacity of the phr1 and sultr1;3 mutants was reduced compared to WT and the sultr2;1 mutants. These results showed that the transcription factor PHR1 up-regulated the expression of SULTR1;3, and exercised a positive control on sulfate inter-organ distribution mediated by SULTR1;3 upon Pi starvation.
Pi starvation alters the expression of the sulfate transporter genes
In order to determine the effect of Pi deficiency on sulfate distribution in Arabidopsis, plants were grown in medium with high Pi (1 mM) for 7 days followed by growth in medium with low Pi (10 μM) for an additional 4 days. Shoots and roots were collected separately and Pi and sulfate contents were determined. As expected, Pi starvation led to a decrease in Pi content in shoots and roots (Figure 1). In contrast, although the sulfate concentration increased 1.8-fold in roots, it decreased 1.4-fold in shoots (Figure 1). This result implies the existence of a process modifying plant root to shoot sulfate distribution under Pi deficiency.
In Arabidopsis, three proteins have been implicated in long-distance sulfate transport between the roots and shoots. SULTR1;3 is involved in the transfer of sulfate from shoot to root , and SULTR3;5 modulates sulfate transport from root to shoot, potentially via its cooperation with SULTR2;1 . Transcript abundance was thus first determined by quantitative RT-PCR for these corresponding genes in shoots and roots of plants grown in media with low and high Pi or sulfate (Figure 2a, c, g). Expression of the SQD1 gene, involved in sulfolipid biosynthesis, was also included as a control, since this gene has been previously reported to be up-regulated by Pi deficiency [1, 25, 30]. The SULTR1;3 transcript was strongly increased in both roots and shoots of Pi-deficient plants, while it was only weakly induced in roots of sulfate-deficient plants (Figure 2a). Transcript abundance of SULTR2;1 showed a weak increase only in roots under Pi deprivation, and a moderate increase in roots under sulfate deprivation (Figure 2c). There was no increase in SULTR3;5 expression under both Pi and sulfate deficiency (data not shown), while SQD1 expression was unchanged under sulfate deficiency but increased in both shoots and roots under Pi deficiency (Figure 2g).
To explore whether expression of any other member of the SULTR gene family was increased by Pi deficiency, microarray data previously generated from Pi-deficient plants were first analyzed [38, 39, 44, 45]. Only SULTR3;4 was consistently induced in Pi-deficient plants in these studies. Analysis by Q-RT-PCR showed that SULTR3;4 expression increased moderately under Pi deficiency in both roots and shoots, while there was no change in expression under sulfate deficiency (Figure 2e).
Gene expression was examined in the pho1 mutant (deficient in the transfer of Pi from roots to shoots ) to investigate whether the increase in transcript levels under Pi deficiency could be controlled by the local level of intracellular Pi as opposed to a systemic response to Pi status emanating from the roots. When grown in complete medium, pho1 mutant shoots are Pi-deficient but roots are Pi-sufficient . While expression of SULTR1;3, SULTR2;1, SULTR3;4 and SQD1 in roots of pho1 plants was not induced, shoots still over-expressed SULTR1;3, SULTR3;4 and SQD1 (Figure 2a, c, e, g). These results indicate that the expression of SULTR1;3, SULTR3;4 and SQD1 was regulated at least partially by the local tissue Pi content instead of a systemic signal initiated in the roots.
Transcript levels were further examined in plants grown in Pi-deficient medium supplemented with 1 mM phosphite. Phosphite is a reduced analogue of Pi that is readily absorbed but neither oxidized nor metabolized by plants. Studies in several plants have shown that numerous molecular and developmental responses to Pi limitations are repressed by phosphite, indicating that phosphite interferes specifically with early events involved in Pi sensing and signaling, including responses typically associated with local Pi sensing or long-distance signaling [47–50]. While addition of phosphite attenuated the induction of SULTR1;3, SULTR3;4 and SQD1 by Pi deficiency in shoot and roots, the same treatment did not lead to decreased SULTR2;1 expression (Figure 2a, c, e, g).
PHR1 regulates the expression of SULTR1;3, SULTR2;1 and SULTR3;4
Among the genes involved in sulfur metabolism, only SQD1 and SQD2, involved in sulfolipid biosynthesis, have been reported to contain the PHR1-binding motif P1BS (GNATATNC) within their promoter [25, 29]. Analysis of the Arabidopsis genome for the P1BS motif within the 500-bp 5'-upstream regulatory sequences identified 3305 genes predicted to contain at least one putative PHR1-binding site. Among this set, only the SULTR2;1 and SULTR1;3 genes were identified as additional genes involved in sulfur metabolism that contained a sequence similar to the P1BS motif. The motifs GGATATTC and GGATATAC are found 432 and 297 bp upstream of the start codon of the SULTR1;3 and SULTR2;1 genes, respectively (Figure 3a). Although induction of SULTR1;3 in Pi-deficient roots and shoots still occurred in the phr1 mutant, it was strongly reduced in both tissues compared to WT plants (Figure 2a, b). A similar level of attenuation without complete loss of induction by Pi deficiency has also been observed for IPS1 and several other genes containing a P1BS sequence and is likely explained by the presence of a functional homolog of PHR1 named PHR1-like (PHL) [25, 37]. As in the case of WT plants, addition of phosphite to Pi-deficient phr1 mutant led to the absence of induction of SULTR1;3 under Pi deficiency (Figure 2b). In contrast to SULTR1;3, the SULTR2;1 expression was higher, particularly in shoots, of Pi-deficient phr1 plants as compared to WT plants, when treated with or without phosphite (Figure 2c, d). A further increase in SULTR3;4 expression in the Pi-deficient phr1 mutant compared to WT was also observed in shoots (Figure 2e, f). However, as observed in WT plants, addition of phosphite abolished any induction of SULTR3;4 by Pi deficiency in phr1 (Figure 2f). There was strong attenuation of SQD1 expression in Pi-deficient plants in the phr1 mutant, both with and without phosphite (Figure 2g, h).
Although the Pi content in shoots of the phr1 mutant was slightly lower than WT for plants grown under Pi-sufficient conditions, consistent with the study of Rubio et al. , there was no significant difference between WT and phr1 in the contents of sulfate or Pi for plants grown under Pi-deficient conditions (Figure 1). These results indicate that the changes in gene expression in the phr1 mutant under Pi-deficient conditions were not due to changes in Pi or sulfate levels in the phr1 mutant compared to WT. Altogether, these results reveal that PHR1 had a positive effect on the expression of SULTR1;3 upon Pi deficiency, but a negative effect on the expression of SULTR2;1 and SULTR3;4.
PHR1 contributes to shoot-to-root sulfate transport
A potential functional role of PHR1 in sulfate homeostasis was assessed by examining the root-to-shoot and shoot-to-root sulfate transfer in the phr1 mutant in comparison to the sultr1;3 and sultr2;1 single mutants and WT plants. T-DNA insertion mutants were isolated for the SULTR1;3 and SULTR2;1 genes (Figure 3a), and the absence of gene expression in homozygous mutants was confirmed by RT-PCR (Figure 3b). There were no notable differences in growth in fertilized soil of the sultr1;3 and sultr2;1 mutants in comparison to phr1 mutant or WT plants (data not shown).
The amount of sulfate and phosphate in the shoots and roots of the sultr1;3 and sultr2;1 mutants were not significantly different from WT, for plants grown under Pi-sufficient or Pi-deficient conditions (Figure 1). The capacity of the mutant lines to transfer radiolabeled sulfate (35S) from roots to shoots and vice-versa was determined for plants grown in media with high or low Pi (Table 1). There were no significant differences in the root-to-shoot sulfate transfer among all genotypes tested, for both Pi treatments. The movement of 35S-labeled sulfate from the shoot to root was not significantly different between WT and the various mutants under Pi-sufficient conditions; however, there was a significant reduction under Pi-deficiency for both phr1 and sultr1;3 mutants compared to Pi-deficient WT and the sultr2;1 mutant (Table 1).
Pi plays a central role in numerous aspects of plant metabolism, and Pi deficiency has profound effects on numerous metabolic pathways as well as on gene expression [38, 39, 44, 51]. These changes in gene expression are expected to help plants adapt to Pi deprivation and adjust their metabolism to sustain growth and ensure survival. Several of the metabolic adjustments triggered by Pi deficiency are expected to have a direct effect on sulfate acquisition and use; e.g. Pi deficiency leads to the replacement of phospholipids by sulfolipid and galactolipids, as well to an increase in glutathione level [1, 52]. Although it is expected that a certain level of coordination and crosstalk must exist between pathways involved in Pi and sulfate transport and homeostasis in plants, important players acting in this coordination remain to be clearly identified.
This current work shows that SULTR1;3, SULTR2;1 and SULTR3;4 genes are up-regulated by Pi deficiency. The pattern of expression of these genes in the pho1 mutant, with basal level of expression in Pi-sufficient roots but overexpression in Pi-deficient shoots, suggests that the response of these genes was mainly associated with a local perception of Pi deficiency. A similar pattern of expression for several Pi deficiency-responsive genes in the pho1 mutant was recently described . Induction of SULTR1;3 and SULTR3;4, by Pi deficiency was suppressed by the Pi analogue phosphite. Studies in several plants have shown that numerous molecular and developmental responses to Pi limitations are repressed by phosphite, indicating that phosphite interferes specifically with early events involved in Pi sensing and signaling, including responses typically associated with local Pi sensing or long-distance signaling [47–50]. The influence of phosphite on SULTR1;3 and SULTR3;4 expression during Pi deficiency places these genes under the influence of this major Pi signal-transduction pathway. However, genes have also been previously identified which are induced by Pi starvation but not suppressed by phosphite, such as the Arabidopsis PHO1 and PHO1;H10 [30, 54], indicating the presence of several distinct Pi deficiency signal-transduction pathways, including phosphite-sensitive and phosphite-insensitive pathways. Our data show that SULTR2;1 belongs to this group of Pi-inducible genes not responding to phosphite.
The present study identified an important role for both SULTR1;3 and PHR1 in the regulation of sulfate inter-organ flux upon Pi starvation in Arabidopsis. PHR1 is a transcription factor that positively regulates the expression of numerous genes upon Pi deficiency and that forms, along with PHO2 and the microRNA miR399, an important branch in the long-distance Pi signaling pathway [25, 29, 32–35]. SULTR1;3 has previously been identified as a high-affinity sulfate transporter expressed in sieve-element-companion-cell complexes of the phloem in cotyledons and roots and involved in sulfate transport from source to sink . Yoshimoto et al.  revealed a small but significant decrease in shoot-to-root sulfate transport in the sultr1;3 mutant under nutrient sufficient conditions; however, in our experiment there was no such significant difference under Pi-sufficient conditions. The experiments reported by Yoshimoto et al.  were performed with a sultr1;3 mutant in a Wassilewskija ecotype while the present study was performed with mutants in the Columbia ecotype. The use of different ecotypes or perhaps differences in culture media composition or plant age could explain the discrepency. Nevertheless, the current study showed that shoot-to-root sulfate transfer was reduced in the Pi-deficient sultr1;3 mutant compared to WT, thus confirming the role of SULTR1;3 in this process. Importantly, the phr1 mutant also showed a decrease in shoot-to-root sulfate transfer in Pi-deficient plants relative to WT, revealing the importance of this Pi-signaling transcription factor in the source-sink sulfate distribution. The fact that the reduction in shoot-to-root sulfate transfer observed in the phr1 mutant was slightly less compared to the sultr1;3 mutant was likely due to the fact that while the sultr1;3 mutant completely abolished expression of the protein, some level of SULTR1;3 expression still remained in the phr1 mutant despite the attenuation in SULTR1;3 expression under Pi deficiency. Altogether, these results bring new insights to the regulation and the function of SULTR1;3 in Pi-deficient plants and identify PHR1 as an important regulator of SULTR1;3.
It is interesting to note that while SQD1 and SQD2, two genes involved in the replacement of phospholipids by sulfolipids, have been identified as containing a PHR1-binding site in their promoter and are up-regulated by Pi deficiency in a PHR1-dependant manner [29, 30], the lipid composition in the phr1 mutant indicated that PHR1 had no significant impact on lipid composition in Pi-deficient plants . Thus, while the phr1 mutant had previously been shown to be altered in various aspects of Pi metabolism, including Pi allocation between roots and shoots and anthocyanin accumulation, no functional implication of PHR1 in sulfur metabolism has been described [25, 31]. In contrast, the present study showed that the implication of PHR1 in the crosstalk between Pi and the sulfate signaling pathway went beyond the regulation of gene expression and that it had an important physiological effect on plant sulfate transport. It is difficult at this point to precisely identify the physiological role of the transport of sulfate from shoots to roots under Pi deficiency. Sulfate is required for the synthesis of numerous compounds that could participate in the Pi-deficiency response, e.g. glutathione levels are known to increase with Pi-deficiency . Pi-deficiency has been associated with an over-accumulation of metals (e.g. iron) and of reactive oxygen species, both of which may trigger the need for additional sulfate for the synthesis of phytochelatin and glutathione [56, 57]. It is also possible that the distribution and requirement for sulfate and sulfur-containing compounds may not be homogeneous across the whole root but may be more localized to the cells surrounding the root vascular cylinder. Further studies should thus analyze in more detail the level of sulfate and sulfur-containing compounds in various cell types within the roots.
While the promoter of SULTR2;1 contains a sequence homologous to the PHR1-binding site, induction of SULTR2;1 by Pi deficiency was not repressed in the phr1 mutant. A similar situation was found for the AtPht1;4 gene, in which the PHR1 motif was required for gene expression in roots but not for its induction upon Pi starvation in shoots . Furthermore, removal of one of the two P1BS sites present in the IPS1 gene abolished its response to Pi deficiency, indicating that the presence of a P1BS element was not sufficient to mediate increased gene expression by Pi deficiency . It is possible that SULTR2;1 mRNA abundance is actually more tightly controlled by a different signaling pathway, notably by the action of the transcription factor SLIM1 and miR395, in order to control sulfate transfer in shoots upon Pi starvation [21–23]. In this context, it was recently shown that miR395, a microRNA up-regulated under sulfate deficiency and that targets SULTR2;1, was down-regulated under Pi deficiency, thus potentially contributing to SULTR2;1 overexpression under Pi deficiency . However, the physiological impact of the down-regulation of miR395 on sulfate metabolism in Pi-deficient plants is not known.
Previous microarray studies showed that a large number of genes were down-regulated by Pi deficiency and use of an inducible PHR1 indicated that transcriptional repression could be indirectly controlled by PHR1 . The increased expression of SULTR2;1 and SULTR3;4 in shoots of a Pi-deficient phr1 mutant relative to Pi-deficient WT plants thus fits with this model of PHR1 playing a key role in both the activation and repression of gene expression. Interestingly, for both SULTR2;1 and SULTR3;4, increased expression in the phr1 mutant was largely confined to the shoot. Distinct expression patterns between shoot and root have been previously noted for SULTR2;1 in the context of sulfate deficiency [13, 23] and further extended in the present study to Pi deficiency (Figure 2c). For sulfate deficiency, this pattern could be explained by the action of miR395 on SULTR2;1 expression in shoots but not in roots because of the non-overlapping tissue-specific expression of miR395 and SULTR2;1 in roots . It is thus possible that the distinct response of SULTR2;1 and SULTR3;4 in roots and shoots of the phr1 mutant may also be caused by non-overlapping expression profiles of PHR1 and these SULTR genes in roots.
Although the role of the transcription factor PHR1 in Pi homeostasis was previously demonstrated, the present study revealed that PHR1 also plays an important role in sulfate homeostasis in plants grown under Pi-deficient conditions. PHR1 stimulated the expression of the SULTR1;3 gene under Pi deficiency, and the significance of this regulation was reflected in a decrease in the shoot-to-root sulfate transfer in the phr1 mutant relative to WT. Interestingly, PHR1 also had a repressive role in the expression of the SULTR2;1 and SULTR3;4 genes in shoots but not in roots. A similar repressive mechanism was recently reported for PSR1, the ortholog of PHR1 in the unicellular alga Chlamydomonas reinhardtii. Analysis of the phenotype of the C. reinhardtii psr1 mutant grown under Pi-deficiency showed that in addition to altering the normal acclimation to Pi deprivation, the psr1 mutant had a de-repressed sulfate deficiency response, leading to overexpression of genes involved in sulfate scavenging and assimilation . Considered together, the results on PSR1 in C. reinhardtii and PHR1 in Arabidopsis reveals an unsuspected level of complexity and interconnection in the regulation of sulfate and Pi homeostasis and highlights the evolutionary conservation of the importance of the PSR1/PHR1 transcription factor in these processes.
Plant growth conditions
The Arabidopsis thaliana mutants used in all experiments were of Columbia ecotype genetic background. The phr1 mutant was kindly obtained from Javier Paz-Ares (CSIC, Madrid) and was previously described . Plants were germinated and grown on agar-solidified media. The complete nutrient medium contained 0.5 mM KNO3, 1 mM MgSO4, 1 mM KH2PO4, 0.25 mM Ca(NO3)2, l00 μM NaFeEDTA, 30 μM H3BO3, l0 μM MnCl2, l μM CuCl2, 1 μM ZnCl2, 0.1 μM (NH4)6Mo7O24, and 50 μM KCl. Sulfate- or Pi-deficient media were made by replacing 1 mM MgSO4 or 1 mM KH2PO4 by 1 mM MgCl2 or 1 mM KCl, respectively. Seeds were put on medium-containing plates and left at 4°C in darkness for stratification for 2 days. Plates were then transferred to a growth chamber under the following environmental conditions: light/dark cycle of 8/16 h, light intensity of 250 μmol·m-2·s-1 and temperature of 24/20°C. Day one of growth is defined as the first day of exposure of stratified seeds to light.
Identification of genes containing the P1BS cis-acting element
A custom Python script (available at http://www.unil.ch/dbmv/page12541_en.html) was used to search for the P1BS cis-acting element (GNATATNC) within the 500-bp 5'-upstream regulatory sequences of 33,282 Arabidopsis gene models in the TAIR dataset (TAIR8_upstream_500_20080228) .
Real-Time Quantitative RT-PCR
Total RNA was extracted from frozen shoot and root tissues using the Plant RNeasy extraction kit (Qiagen, http://www.qiagen.com). Any residual genomic DNA was eliminated using a RNAse-free DNAse I (Fermentas, http://www.fermentas.com). Total RNA was quantified with a NanoDrop spectrophotometer (Thermo Scientific, http://www.nanodrop.com). Two micrograms of total RNA were reverse transcribed using the SuperscriptIII RT kit (Promega, http://www.promega.com). Quantitative real-time RT-PCR was performed with a Stratagene Mx3000P apparatus http://www.stratagene.com using SYBR green dye technology (BioRad, http://www.bio-rad.com). The primers used in this work (Additional file 1, Table S1) had an efficiency of amplification ≥ 1.85. PCR reactions were performed in a final volume of 25 μL containing 300 nM each of the forward and reverse primers, 12.5 μL of the SYBR green master mix and 5 μL of a 1:50 cDNA dilution. All PCR reactions were performed in triplicate. For each gene, the relative amount of calculated mRNA was normalized to the level of the control gene ubiquitin mRNA (UBQ10: At4g05320) and expressed as relative values against WT plants grown in complete (+Pi and +sulfate) medium. Reactions were performed in an optical tube (Stratagene) covered with an optical cap (Stratagene). Samples were submitted to 95°C for 15 min, then to 45 cycles of 95°C for 15 s followed by 55°C for 30 s and 72°C for 30 s. Data were analyzed using the MxPro™ software (Stratagene). The specificity of the amplified PCR products and quantification of the relative transcripts levels was performed using the comparative CT method [20, 61].
Identification of the sultr1;3 and sultr2;1mutants
The T-DNA insertion mutants sultr1;3 (N669442) and sultr2;1 (N655235) were from the SALK collection  and obtained from the European Arabidopsis Stock Centre http://www.arabidopsis.info. The homozygous mutants were identified and confirmed by PCR using oligonucleotides (see Additional file 1, Table S1, for the sequences).
Sulfate uptake and transfer measurements
Sulfate transport measurements were performed using whole plants grown in vitro for 7 days in medium containing 1 mM PO42- followed by 4 days in medium without PO42-. For root-to-shoot measurements, roots of whole plants were placed in a 10 μM Na2SO4 solution at pH 5.0 in the presence of 1 μCi/mL of the radiotracer 35S-Na2SO4 (PerkinElmer; http://www.perkinelmer.com) for 90 min. Plants were then washed in an ice-cold 5 mM Na2SO4 solution, and then shoots and roots were harvested separately, blotted with paper towel and the radioactivity measured by scintillation counting. Root-to-shoot sulfate transport was expressed as the percentage of radioactivity located in the shoot over the total amount of radioactivity in the whole plant.
Shoot-to-root sulfate influx measurements were performed using 11-day old plants with similar conditions as described for root measurements, except that the 2 μCi/mL of the radiotracer 35S-Na2SO4 and 0.01% Triton X-100 were deposited on the leaves. Shoot-to-root sulfate transport was expressed as the percentage of radioactivity located in the roots over the total amount of radioactivity in the whole plant.
Phosphate and sulfate measurements
Anion measurements were performed as described by Rouached et al. . Briefly, weighed fresh shoots and roots were ground separately into powder in liquid nitrogen and extracted in water by incubation for 30 min at 70°C. The extract was centrifuged, and the supernatant filtered through a 0.45-μm filter unit. Ion concentration was determined by High-Pressure Ionic Chromatography (ICS-2100 apparatus; Dionex, http://www.dionex.com) using the AS19 anion exchanging column (Dionex) and a KOH gradient. Identification and quantification of Pi and sulfate were performed by comparison of the retention times and peak areas with standards and integrated using the Chromeleon software (Dionex).
Essigmann B, Guler S, Narang RA, Linke D, Benning C: Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA. 1998, 95: 1950-1955. 10.1073/pnas.95.4.1950.
Hartel H, Essigmann B, Lokstein H, Hoffmann-Benning S, Peters-Kottig M, Benning C: The phospholipid-deficient pho1 mutant of Arabidopsis thaliana is affected in the organization, but not in the light acclimation, of the thylakoid membrane. Biochim Biophys Acta. 1998, 1415: 205-218. 10.1016/S0005-2736(98)00197-7.
Sugimoto K, Sato N, Tsuzuki M: Utilization of a chloroplast membrane sulfolipid as a major internal sulfur source for protein synthesis in the early phase of sulfur starvation in Chlamydomonas reinhardtii. FEBS Lett. 2007, 581: 4519-4522. 10.1016/j.febslet.2007.08.035.
Yu B, Xu C, Benning C: Arabidopsis disrupted in SQD2 encoding sulfolipid synthase is impaired in phosphate-limited growth. Proc Natl Acad Sci USA. 2002, 99: 5732-5737. 10.1073/pnas.082696499.
Gojon A, Nacry P, Davidian JC: Root uptake regulation: a central process for NPS homeostasis in plants. Curr Opin Plant Biol. 2009, 12: 328-338. 10.1016/j.pbi.2009.04.015.
Smith FW, Rae AL, Hawkesford MJ: Molecular mechanisms of phosphate and sulphate transport in plants. Biochim Biophys Acta. 2000, 1465: 236-245. 10.1016/S0005-2736(00)00141-3.
Hawkesford MJ, Davidian JC, Grignon C: Sulphate/proton cotransport in plasma-membrane vesicles isolated from roots of Brassica napus L.: increased transport in membranes isolated from sulphur-starved plants. Planta. 1993, 190: 297-307. 10.1007/BF00196957.
Smith FW, Ealing PM, Hawkesford MJ, Clarkson DT: Plant members of a family of sulfate transporters reveal functional subtypes. Proc Natl Acad Sci USA. 1995, 92: 9373-9377. 10.1073/pnas.92.20.9373.
Buchner P, Takahashi H, Hawkesford MJ: Plant sulphate transporters: co-ordination of uptake, intracellular and long-distance transport. J Exp Bot. 2004, 55: 1765-1773. 10.1093/jxb/erh206.
Shibagaki N, Rose A, McDermott J, Fujiwara T, Hayashi H, Yoneyama T, Davies J: Selenate-resistant mutants of Arabidopsis thaliana identify Sultr1;2, a sulfate transporter required for efficient transport of sulfate into roots. Plant J. 2002, 29: 475-486. 10.1046/j.0960-7412.2001.01232.x.
Yoshimoto N, Takahashi H, Smith FW, Yamaya T, Saito K: Two distinct high-affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsis roots. Plant J. 2002, 29: 465-473. 10.1046/j.0960-7412.2001.01231.x.
Yoshimoto N, Inoue E, Saito K, Yamaya T, Takahashi H: Phloem-localizing sulfate transporter, Sultr1;3, mediates re-distribution of sulfur from source to sink organs in Arabidopsis. Plant Physiol. 2003, 131: 1511-1517. 10.1104/pp.014712.
Takahashi H, Watanabe-Takahashi A, Smith FW, Blake-Kalff M, Hawkesford MJ, Saito K: The roles of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis thaliana. Plant J. 2000, 23: 171-182. 10.1046/j.1365-313x.2000.00768.x.
Kataoka T, Hayashi N, Yamaya T, Takahashi H: Root-to-shoot transport of sulfate in Arabidopsis. Evidence for the role of SULTR3;5 as a component of low-affinity sulfate transport system in the root vasculature. Plant Physiol. 2004, 136: 4198-4204. 10.1104/pp.104.045625.
Zuber H, Davidian JC, Aubert G, Aimé D, Baelghazi AM, Lugan R, Heintz D, Wirtz M, Hell R, Thompson R, et al: The seed composition of Arabidopsis mutants for the group 3 sulfate transporters indicates a role in sulfate translocation within developing seeds. Plant Physiol. 2010, 154: 913-926. 10.1104/pp.110.162123.
Kataoka T, Watanabe-Takahashi A, Hayashi N, Ohnishi M, Mimura T, Buchner P, Hawkesford MJ, Yamaya T, Takahashi H: Vacuolar sulfate transporters are essential determinants controlling internal distribution of sulfate in Arabidopsis. Plant Cell. 2004, 16: 2693-2704. 10.1105/tpc.104.023960.
Zuber H, Davidian JC, Wirtz M, Hell R, Belghazi M, Thompson R, Gallardo K: Sultr4;1 mutant seeds of Arabidopsis have an enhanced sulphate content and modified proteome suggesting metabolic adaptations to altered sulphate compartmentalization. BMC Plant Biol. 2010, 10: 78-10.1186/1471-2229-10-78.
Tomatsu H, Takano J, Takahashi H, Watanabe-Takahashi A, Shibagaki N, Fujiwara T: An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil. Proc Natl Acad Sci USA. 2007, 104: 18807-18812. 10.1073/pnas.0706373104.
Davidian JC, Kopriva S: Regulation of sulfate uptake and assimilation- the same or not the same?. Mol Plant. 2010, 3: 314-325. 10.1093/mp/ssq001.
Rouached H, Wirtz M, Alary R, Hell R, Arpat AB, Davidian JC, Fourcroy P, Berthomieu P: Differential regulation of the expression of two high-affinity sulfate transporters, SULTR1.1 and SULTR1.2, in Arabidopsis. Plant Physiol. 2008, 147: 897-911. 10.1104/pp.108.118612.
Maruyama-Nakashita A, Nakamura Y, Tohge T, Saito K, Takahashi H: Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. Plant Cell. 2006, 18: 3235-3251. 10.1105/tpc.106.046458.
Jones-Rhoades MW, Bartel DP: Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol Cell. 2004, 14: 787-799. 10.1016/j.molcel.2004.05.027.
Kawashima CG, Yoshimoto N, Maruyama-Nakashita A, Tsuchiya YN, Saito K, Takahashi H, Dalmay T: Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. Plant J. 2009, 57: 313-321. 10.1111/j.1365-313X.2008.03690.x.
Liang G, Yang F, Yu D: MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana. Plant J. 2010, 62: 1046-1057.
Rubio V, Linhares F, Solano R, Martin AC, Iglesias J, Leyva A, Paz-Ares J: A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae. Genes Dev. 2001, 15: 2122-2133. 10.1101/gad.204401.
Devaiah BN, Karthikeyan AS, Raghothama KG: WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiol. 2007, 143: 1789-1801. 10.1104/pp.106.093971.
Devaiah BN, Nagarajan VK, Raghothama KG: Phosphate homeostasis and root development in Arabidopsis are synchronized by the zinc finger transcription factor ZAT6. Plant Physiol. 2007, 145: 147-159. 10.1104/pp.107.101691.
Devaiah BN, Madhuvanthi R, Karthikeyan AS, Raghothama KG: Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis. Mol Plant. 2009, 2: 43-58. 10.1093/mp/ssn081.
Franco-Zorrilla JM, Gonzalez E, Bustos R, Linhares F, Leyva A, Paz-Ares J: The transcriptional control of plant responses to phosphate limitation. J Exp Bot. 2004, 55: 285-293. 10.1093/jxb/erh009.
Stefanovic A, Ribot C, Rouached H, Wang Y, Chong J, Belbahri L, Delessert S, Poirier Y: Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. Plant J. 2007, 50: 982-994. 10.1111/j.1365-313X.2007.03108.x.
Nilsson L, Muller R, Nielsen TH: Increased expression of the MYB-related transcription factor, PHR1, leads to enhanced phosphate uptake in Arabidopsis thaliana. Plant Cell Environ. 2007, 30: 1499-1512. 10.1111/j.1365-3040.2007.01734.x.
Aung K, Lin SI, Wu CC, Huang YT, Su CL, Chiou TJ: pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a miR399 target gene. Plant Physiol. 2006, 141: 1000-1011. 10.1104/pp.106.078063.
Bari RP, Pant BD, Stitt M, Scheible WR: PHO2, micro RNA399 and PHR1 define a phosphate signalling pathway in plants. Plant Physiol. 2006, 141: 988-999. 10.1104/pp.106.079707.
Lin SI, Chiang SF, Lin WY, Chen JW, Tseng CY, Wu PC, Chiou TJ: Regulatory network of microRNA399 and PHO2 by systemic signaling. Plant Physiol. 2008, 147: 732-746. 10.1104/pp.108.116269.
Pant BD, Buhtz A, Kehr J, Scheible WR: MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis. Plant J. 2008, 53: 731-738. 10.1111/j.1365-313X.2007.03363.x.
Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan AS, Raghothama KG, Baek D, Koo YD, Jin JB, Bressan RA, et al: The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci USA. 2005, 102: 7760-7765. 10.1073/pnas.0500778102.
Bustos R, G C, Linhares F, Puga MI, Rubio V, Pérez-Pérez J, Solano R, Leyva A, Paz-Ares J: A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis. PloS Genet. 2010, 6: e1001102-10.1371/journal.pgen.1001102.
Misson J, Raghothama KG, Jain A, Jouhet J, Block MA, Bligny R, Ortet P, Creff A, Somerville S, Rolland N, et al: A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proc Natl Acad Sci USA. 2005, 102: 11934-11939. 10.1073/pnas.0505266102.
Müller R, Morant M, Jarmer H, Nilsson L, Nielsen TH: Genome-wide analysis of the Arabidopsis leaf transcriptome reveals interaction of phosphate and sugar metabolism. Plant Physiol. 2007, 143: 156-171.
Thibaud MC, Arrighi JF, Bayle V, Chiazenza S, Creff A, Bustos R, Paz-Ares J, Poirier Y, Nussaume L: Dissection of local and systemic transcriptional responses to phosphate starvation in Arabidopsis. Plant J. 2010, 64: 775-789. 10.1111/j.1365-313X.2010.04375.x.
Moseley JL, Gonzalez-Ballester D, Pootakham W, Bailey S, Grossman AR: Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics. 2009, 181: 889-905. 10.1534/genetics.108.099382.
O'Connell KF, Baker RE: Possible cross-regulation of phosphate and sulfate metabolism in Saccharomyces cerevisiae. Genetics. 1992, 132: 63-73.
Saldanha AJ, Brauer MJ, Botstein D: Nutritional homeostasis in batch and steady-state culture of yeast. Mol Biol Cell. 2004, 15: 4089-4104. 10.1091/mbc.E04-04-0306.
Morcuende R, Bari RP, Gibon Y, KZheng W, Datt Pant B, Bläsing O, Usadel B, Czechowski T, Udvardi MK, Stitt M, et al: Genome-wide reprogramming of metabolism and regulatory networks of Arabidopsis in response to phosphorus. Plant Cell Environ. 2007, 30: 85-112. 10.1111/j.1365-3040.2006.01608.x.
Hammond JP, Bennett MJ, Bowen HC, Broadley MR, Eastwood DC, May ST, Rahn C, Swarup R, Woolaway KE, White PJ: Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants. Plant Physiol. 2003, 132: 578-586. 10.1104/pp.103.020941.
Poirier Y, Thoma S, Somerville C, Schiefelbein J: A mutant of Arabidopsis deficient in xylem loading of phosphate. Plant Physiol. 1991, 97: 1087-1093. 10.1104/pp.97.3.1087.
Carswell C, Grant BR, Theodorou ME, Harris J, Niere JO, Plaxton WC: The fungicide phosphonate disrupts the phosphate-starvation response in Brassica nigra seedlings. Plant Physiol. 1996, 110: 105-110.
Carswell MC, Grant BR, Plaxton WC: Disruption of the phosphate-starvation response of oilseed rape suspension cells by the fungicide phosphonate. Planta. 1997, 203: 67-74.
Ticconi CA, Delatorre CA, Abel S: Attenuation of phosphate starvation responses by phosphite in Arabidopsis. Plant Physiol. 2001, 127: 963-972. 10.1104/pp.010396.
Varadarajan DK, Karthikeyan AS, Matilda PD, Raghothama KG: Phosphite, an analog of phosphate, suppresses the coordinated expression of genes under phosphate starvation. Plant Physiol. 2002, 129: 1232-1240. 10.1104/pp.010835.
Poirier Y, Bucher M: Phosphate transport and homeostasis in Arabidopsis. The Arabidopsis Book. Edited by: Somerville CR, Meyerowitz EM. American Society of Plant Biologists, Rockville, MD, [http://www.bioone.org/doi/full/10.1043/tab.00024]
Kandlbinder A, Finkemeier I, Wormuth D, Hanitzsch M, Dietz K: The antioxidant status of photosynthesizing leaves under nutrient deficiency: redox regulation, gene expression and antioxidant activity in Arabidopsis thaliana. Physiol Plant. 2004, 120: 63-73. 10.1111/j.0031-9317.2004.0272.x.
Rouached H, Stefanovic A, Secco D, Arpat B, Gout E, Bligny R, Poirier Y: Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis. Plant J. 2010,
Ribot C, Wang Y, Poirier Y: Expression analyses of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the response to auxin, cytokinin and abscisic acid. Planta. 2008, 227: 1025-1036. 10.1007/s00425-007-0677-x.
Gaude N, Nakamura Y, Scheible WR, Ohta H, Dormann P: Phospholipase C5 (NPC5) is involved in galactolipid accumulation during phosphate limitation in leaves of Arabidopsis. Plant J. 2008, 56: 28-39. 10.1111/j.1365-313X.2008.03582.x.
Hirsch J, Marin E, Floriani M, Chiarenza S, Richaud P, Nussaume L, Thibaud MC: Phosphate deficiency promotes modification of iron distribution in Arabidopsis plants. Biochimie. 2006, 88: 1767-1771. 10.1016/j.biochi.2006.05.007.
Nocito FF, Pirovano L, Cocucci M, Sacchi GA: Cadmium-induced sulfate uptake in maize roots. Plant Physiol. 2002, 129: 1872-1879. 10.1104/pp.002659.
Karthikeyan A, Ballachanda D, Raghothama K: Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiol Plant. 2009, 136: 10-18. 10.1111/j.1399-3054.2009.01207.x.
Hsieh L, Lin S, Shih A, Chen J, Lin W, Tseng C, Li W, Chiou T: Uncovering small RNA-mediated responses to phosphate-deficiency in Arabidopsis by deep sequencing. Plant Physiol. 2009, 151: 2120-2132. 10.1104/pp.109.147280.
Swarbreck D, Wilks C, Lamesch P, Berardini TZ, Garcia-Hernandez M, Foerster H, Li D, Meyer T, Muller R, Ploetz L, et al: The Arabidopsis Information Resource (TAIR): gene structure and function annotation. Nucleic Acid Res. 2008, 36: D1009-D1014. 10.1093/nar/gkm965.
Schmittgen TD, Livak KJ: Analyzing real-time PCR data by the comparative C-T method. Nat Protoc. 2008, 3: 1101-1108. 10.1038/nprot.2008.73.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, et al: Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003, 301: 653-657. 10.1126/science.1086391.
The authors are grateful to Javier Paz-Ares (CSIC, Madrid) for providing seeds of the phr1 mutant. This work was funded by a grant to YP from the 'Fonds National Suisse de la Recherche Scientifique' (Grant 3100A0-122493).
HR conceived the study; HR, DS and BA performed all experiments; and HR and YP analyzed the data and wrote the paper. All authors discussed the results, read and approved the final manuscript.
About this article
Cite this article
Rouached, H., Secco, D., Arpat, B. et al. The transcription factor PHR1 plays a key role in the regulation of sulfate shoot-to-root flux upon phosphate starvation in Arabidopsis. BMC Plant Biol 11, 19 (2011). https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2229-11-19
- Sulfate Transport
- Sulfate Deficiency
- Sulfate Transfer
- Pho1 Mutant