Figure 6From: The elicitation of a systemic resistance by Pseudomonas putidaBTP1 in tomato involves the stimulation of two lipoxygenase isoformsEvaluation of recombinant TomLOXF produced in E. coli. We verified the production and activity of the recombinant TomLOXF through LOX activity assay (A) and SDS-PAGE and Western blotting with an anti-His-tag antibody (B). Four clones were tested: two clones containing pET28-a plasmid without the TomLoxF insert (P) and two clones containing pET28-a with the TomLoxF insert (F), induced (+) or not (-) by IPTG. M: Page Ruler Plus Prestained Protein Ladder (Fermentas).Back to article page