Figure 4

Phenotypes of rice seedlings under salt stress. (A) OsCYP2 transgenic rice lines showed salt tolerant phenotypes. Ten-day-old rice seedlings were treated with 200 mM NaCl. After 5 days, phenotypes of rice seedlings were observed. WT represents the wild-type seedling, Aichi ashahi that was used as a reference rice cultivar. (B) Western blot showed OsCYP2 overexpression in two OsCYP2 transgenic lines (OE1 and OE2). The housekeeping protein, Actin (Os03g0718100), was used as equal loading control. (C) Real time PCR exhibited differential expression pattern of OsCYP2 between WT and OsCYP2 transgenic lines (OE1 and OE2) under salt stress. Ten-day-old rice seedlings were treated for 1 d with 200 mM NaCl. An actin gene, Os03g0718100, was used as internal standard. (D) The altered activity of PPIase was found in WT and OsCYP2 transgenic lines (OE1 and OE2) under salt stress. Ten-day-old rice seedlings were treated for 1 d with 200 mM NaCl. Cyclosporin A (CsA) was able to partly inhibit the activity of PPIase.