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Table 3 Oligonucletotides used for cloning

From: Uncharacterized conserved motifs outside the HD-Zip domain in HD-Zip subfamily I transcription factors; a potential source of functional diversity

Oligonucleotide name

Sequence

Restriction sites

construct

Transfl

5'-gCggATCCACCATgTCTTTCAACAAgTA-3'

BamHI

H4-H1 cloning

H1CDS-R

5'-ggggAgCTCTCAATTgAATTgTggTTgTTCC-3'

SacI

H4-H1 cloning

H1*H4F

5'-gTTggAggTgTAAAAAATAgggAgCCAgC-3'

 

H4-H1 cloning

H1*H4R

5'-TATTTTTTTAgCACCTCCAACTgATTgAgTAgg-3'

 

H4-H1 cloning

H4-WCT-R

5'-CCCgAgTCTCTATTCTTCACCgCTgCCAC-3'

SacI

H4WCT cloning

H1-WCT-R

5'-ggCgAgCTCTCATCCTTCTgTTTCTTTTATgTTgAgg-3'

SacI

H1WCT cloning

H1atF

5'-gggggATCCgCTgATgACTTgCACTggAATggC-3'

BamHI

H1WCT cloning

H1qR

5'-CCAACCATggCCAAAACCCTg-3'

 

H1 qRT-PCR

H1qF

5'-ggCCggCAgATCATCAACTTC-3'

 

H1 qRT-PCR

H4qR

5'-gCCgAgTCTTAgAACTCCAACCACTTTTg-3'

 

H4 qRT-PCR

H4qF

5'-CgCgATCAAAgTCgAggCAgATTg-3'

 

H4 qRT-PCR

H4quimqF

5'-CgCgATCAAAgTCgAggCAgATTg-3'

 

H4-H1 qRT-PCR

H1AsTH4R

5'-TCCTTCTgTTTCTTTTATgTTgAgg-3'

 

H4-H1 qRT-PCR

UBCQPCR-R

5'-CAgTggACTCgTACTTgTTCTTgT-3'

 

Genomic DNA

UBC9GENOM-F

5'-gTTTTggAAATgTTgACAggAC-3'

 

Genomic DNA

ATHB1F

5' gCggAATTCATggAATCCAATTCgTTTTTC 3'

EcoRI

ATHB1 and ATHB1WCT cloning

ATHB1R

5' gCgggATCCTAAggCCATCCCCAgAAAg 3'

BamH1

ATHB1 cloning

ATHB1WCTR

5' gCggTCgACTACTCTTgTTTgCCCTgAAgC 3'

Sal1

ATHB1WCT cloning

ATHB1CTF

5' gCggAATTCCAAgAgACAgCTAATgAACCA 3'

EcoRI

ATHB1 CTR cloning

  1. Sequence of oligonucleotides used in PCR reactions to perform constructs or cDNA quantifying. In bold, the overlapping sequences for chimerical constructs and underlined the restriction sites used for cloning.