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Table 1 SERK and SERKL genes identified in M. truncatula

From: Characterisation of the legume SERK-NIKgene superfamily including splice variants: Implications for development and defence

Gene name

Genomic identifier

Chr

TC/EST

identified

Current TC number

No of ESTs on DFCI

Deg

PCR

Matching

probeset ID

on MtGI

Chr Pos

(Kbp)

Gene loci

(Medtr-)

SV

GenBank

Number

Protein length

MW

pI

MtSERK1

AY162177

0

 

TC142011

10

yes

Mtr.43625.1.S1_at

   

AY162176

627

69140.3

5.48

MtSERK2

AC195567 AC187356

2

TC100619

TC97176

TC150247

5

yes

Mtr.37421.1.S1_at

1603.3-1609.6

2g008470

2g008480

 

HM640001

619

68538.8

5.47

MtSERK3

AC195567

AC187356

2

  

0

no

none present

1610.0 - 1616.1

2g008490

2g008500

SV1

HM640008

586

65127.2

5.12

          

SV2

HM769882

271

29246.0

4.98

          

SV3

HM769883

562

62537.2

5.20

          

SV4

HM769884

247

26656.1

5.22

          

SV5

HM769885

154

16964.2

4.59

          

SV6

HM769886

154

16964.2

4.59

          

SV7

HM769887

154

16964.2

4.59

MtSERK4

AC195567

AC187356

2

  

0

no

none present

1615.7-1621.4

2g008510

 

HM640002

615

67882.3

5.50

MtSERK5

AC195567

2

TC104947

TC110830

TC155497

TC151948

8

yes

Mtr.39468.1.S1_at

Mtr.11713.1.S1_at

1622.7-1628.9

2g008520

 

HM640003

620

68615.9

5.61

MtSERK6

AC195567

2

  

0

no

none present

1629.2-1636.2

2g008530

2g008540

 

HM640004

642

70720.3

5.41

MtSERKL1

AC126784

8

CB891120

TC143055

4

no

Mtr.15874.1.S1_s_at Mtr.15874.1.S1_at

35000.0-35005.0

8g144660

 

HM640005

640

70293.2

6.66

MtSERKL2

CT025841

5

TC109616

TC150718

5

no

Mtr.41552.1.S1_at

14476.6-14481.4

5g035120

 

HM640006

625

69142.3

6.86

MtSERKL3

CT967306

3

TC97017

TC166655

10

no

Mtr.44258.1.S1_at

24728.8-24736.2

3g101870

 

HM640007

609

68019.8

5.64

  1. Summary of SERK and SERKL genes in M. truncatula including splice variants (SV1-7) of MtSERK3. Gene name refers to the final name given to each gene. The genomic identifier is the GenBank number of the genomic sequence containing each gene. Chr is chromosome number. TC/EST identified refers to any matching TC or EST sequence found on the DFCI Medicago gene index at the time the eight new genes were first identified. These numbers have since been updated and sometimes divided into separate sequences. Current TC number shows the current corresponding TC numbers for each sequence. No. of ESTs on DFCI is the number of ESTs used to compile each TC sequence on the DFCI Medicago gene index. Detected on degenerate PCR indicates which sequences we found using that technique. Matching probeset ID on MtGI indicates the corresponding probeset on the M. truncatula Gene Expression Atlas. Chr Pos is the gene position in kilobase pairs (Kbp) on each chromosome established from CViT blast searches. Gene loci indicates the gene locus number/s present at each site. Splice variant (SV) numbers of the 7 MtSERK3 SVs are given. GenBank numbers apply to full-length mRNA sequences deposited on the NCBI database. Length, molecular weight (MW) and pI values of predicted protein sequences are shown.