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Figure 4 | BMC Plant Biology

Figure 4

From: Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper

Figure 4

RT-PCR detection of TRV1 and TRV2 viral RNAs. A) In small fruits of approximately 10 DAA of infiltrated plant 1, with the changed shape phenotype and infiltrated plant 3, with the typical round shape phenotype, 9 weeks after infiltration. White - photobleached leaves from pepper plants infiltrated with pTRV2-NbPds were used as the control for the verification of the PCR success. TRV's transcription is confirmed by the presence of TRV1 and TRV2 transcripts in the infiltrated plant 1, while no TRV is detected in infiltrated plant 3. B) In leaves of the wild type (WT) - not infiltrated plant, of mock plant 1 and mock plant 2, of infiltrated plant 1, with the changed shape pheonotype approx. 11 weeks after infiltration. TRV1 transcripts are detected in mock 2 and infiltrated plant 1 but not in mock 1 and the WT. On the other hand, TRV2 transcripts are detected only in mock 2. Again white - photobleached leaves from pepper plants infiltrated with pTRV2-NbPds were used as the control for the verification of the PCR success. Pepper Actin was used for the verification of successful first strand cDNA synthesis. C) In 5 DAA fruits of the WT - not infiltrated plant and of infiltrated plant 1, 16.5 weeks after infiltration. TRV1 but not TRV2 transcripts are detected (as in leaves earlier) in the fruit of the infiltrated plant 1 while no transcripts are detected in the fruit taken from the WT.

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