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Figure 5 | BMC Plant Biology

Figure 5

From: Multiple evidence for the role of an Ovate-like gene in determining fruit shape in pepper

Figure 5

Relative quantitative RT-PCR of CaOvate expression in fruit of 5 DAA from the wild type (WT) and the infiltrated plant 1. First strand cDNA synthesis was accomplished starting from total RNA isolated from both fruits and using random hexamers and reverse transcriptase. This first strand cDNA was used in the PCR using gene-specific primers for CaOvate, one of them designed on the sequence outside the area covered by the construct. The samples from both plants were collected approx. 16.5 weeks after the infiltration which was done when the seedlings were in the stage of cotyledons. Asterisk indicates statistically significant difference (p < .05) of the expression levels of CaOvate in the 5 DAA fruit of the infiltrated plant 1 when compared to expression levels in the 5 DAA fruit of the WT. Pepper Actin was used as a reference gene.

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