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Figure 1 | BMC Plant Biology

Figure 1

From: Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana

Figure 1

Effects of AtHb1 overexpression in Arabidopsis seeds. (A) Northern blot analysis of AtHb1 expression in WT and homozygous transgenic plants (L1-1 and L1-4) at 45 DAG, 25S RNA was used as loading control. (B) RT-PCR analysis of AtHb1 expression in different tissues of L1-1. (C) RT-PCR analysis of AtHb1 expression in siliques of WT and L1-1 under control conditions and moderate hypoxia. (D) Fluorescence detection assay of NO using DAF-2DA. Fluorescence signals (green) indicate NO accumulation.

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