Figure 2
From: Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana
UGT73C6 pro :GUS expression is present in all organs and is developmentally regulated. (a) A homozygous line expressing a UGT73C6 promoter GUS fusion, that showed a characteristic staining pattern, was chosen for histochemical analysis of UGT73C6 pro :GUS expression in different organs and developmental stages. (b) Response of UGT73C5 UGT73C6, DWF4, ROT3 and BR6ox2 expression in eight-day-old whole seedlings to external application of 1 μM 24-epiBL. GAPC2 was used for standardization. The mean and standard deviation of three biological replicates is shown. UGT73C5 and UGT73C6 expression levels are not statistically significantly altered (t-test p-value > 0.05) in treated versus untreated samples, while the expression of DWF4, ROT3 and BR6ox2 is significantly repressed (t-test p-value < 0.01) by 1 μM of 24-epiBL.