Figure 6

Transmission electron micrographs showing the subcellular distribution of glutathione in Col-0 pollen grains. Gold particles bound to glutathione could be found evenly distributed in plastids (P), mitochondria (M), and the cytosol but not in lipid bodies (L), vacuoles (V) and cell walls of pollen grains obtained from Arabidopsis thaliana accession Col-0. Pollen grains were grown on solidified pollen germination medium for 5 hours with either (a) no GSH and BSO (control), (b) 0.1 mM BSO, (c) 1 mM GSH, (d) 0.1 mM BSO and 1 mM GSH, (e) 3 mM GSH, and (f) 3 mM GSH and 0.1 mM BSO prior to fixation. Bars = 1 μm.