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Figure 4 | BMC Plant Biology

Figure 4

From: Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey

Figure 4

Characteristic features of organelle markers and subcellular location of proteins of flower-abundant genes in Onc . Gower Ramsey. A. Mitochondrial marker: the first 29 amino acids of yeast cytochrome c oxidase IV fused with RFP. B. Plastid marker: the targeting sequence (first 79 aa) of the small subunit of tobacco rubisco fused with GFP. C. CFP peroxisome marker: cytoplasmic tail and transmembrane domain of soybean 1, 2-mannosidase I fused with CFP. D. RFP plasma membrane marker: the full length of AtPIP2A, a plasma membrane aquaporin fused with RFP. E. YFP vacuole marker: γ-TIP, an aquaporin of the vacuolar membrane fused with YFP. F. Nuclear marker: NLS domain of VirD2 fused with mCherry. G. YFP: OnTI1: YFP fused with the N-terminus of OnTI1 protein. H. OnTI1::GFP + Mito-RFP: OnTI1::GFP and Mitochondria RFP marker were co-transformed to the cells. I. YFP::OnTI2: YFP fused with the N-terminus of OnTI1 protein. J. YFP::OnTI3: YFP fused with the N-terminus of OnTI3 protein. K. YFP::OnExpansin: YFP fused with the N-terminus of OnExpansin protein. L. YFP::OnDRRP: YFP fused with the N-terminus of OnDRRP protein.

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