Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey

Table 2 Primers used for RT-PCR and BAC screening

Gene	Forward primer	Reverse primer	Clone ID	GenBank No.
UBQ	ACA TTC AGA AGG AGT CAA CCC	CGATGTCGATTTCGATTTCC
OnDRRP	TGAAAAAGAAACCCATCTGCA	GCCCATAGGTGCCAATATTT	P-5-O-22	HQ832781
OnExpansin	ACGCAACTTTCTATGGCGG	AAGCAACCACAGCTCCAAGT	O-1-O-24	HQ832782
OnTI1	ATCACTTTGGCTCTGCTGCTT	TGCCGAGGTCCTCGACTTCCA	J-1-K-16	HQ832783
OnTI2	AAGAAGAACTCCCCACAAGAA	AGGTTGATCGATCGAAGCA	J-1-K-16	HQ832783
OnTI3	ATCACTTTGGCTCTGCTGCTT	AGCAATGAATGACGATCGAC	J-1-K-16	HQ832783
OMADS3	GAGGTATCAGCAAGTTACCG	CGAACGATCTTAATCGACTC	45-3-B-1	HQ832787
OMADS6	AAACCCAGAGTAGTCAGCAG	GTCATATCCCATTGCATGA	73-1-K-8	HQ832788
OMADS8	ATGGAAGGCAGCATGAGAGAAC	AAAGCGTTAGCATTGTTACTTGTTT	AAP-1-C-19	HQ832789
OMADS9	GATAAACCAAAACCTGAGGA	TTTTGTAGGTATCGGTCTGG	L-1-P-13	HQ832790
OnFT	ATTGTAGGACGAGTGATTGG	TACTTGGACTTGGAGCATCT	Q-1-I-4	HQ832784
OnLeafy	TTCCTGGATCTCAACATCAT	TGCTGAAATCCTCAAACTTCA	Orp-2-F-21	HQ832785
OnTFL	TTGTAGTTGGTAGAGTTATAGGAGAAG	ATCAGTCATAATCAGTGTGAAGAAAG	Q-1-B-10	HQ832786

ISSN: 1471-2229