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Table 2 Verification of microarray results using RT-qPCR

From: HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers

Leaf (up-regulated transcripts)

Microarray

RT-qPCR

 

EST/mRNA

Description

Fold

Fold

s.e

EH620344

Arabidopsis thaliana FKF1 (FLAVIN-BINDING, KELCH REPEAT, F BOX 1)

12.45

18.36

2.97

EH615198

Nicotiana tabacum nictaba (NT1) mRNA Jasmonic acid methyl ester and ethylene-induced mRNA

6.41

10.1

2.90

FG156808

Nicotiana tabacum 1-D-deoxyxylulose 5-phosphate synthase (DXS) mRNA

3.61

3.30

0.27

Leaf (down-regulated transcript)

 

AY741503

Nicotiana tabacum S-Adenosyl- L-methionine methyl transferase mRNA (SAMT) (p = 0.067)

0.44

0.35

0.11

Leaf (non-regulated transcripts)

 

EB450395

Arabidopsis thaliana ARPC3 (actin-related protein C3)

1.09

1.00

0.00

X67159

Nicotiana tabacum pectate lyase mRNA

0.98

1.01

0.01

Flower (up-regulated transcripts)

 

EB438380

Solanum lycopersicum Trypsin and protease inhibitor, mRNA

2.86

3.50

0.94

EB683763

Nicotiana tabacum mRNA for P-rich protein NtEIG-C29

2.03

2.10

0.28

FG157361

Nicotiana tabacum mRNA for RAV

2.14

1.60

0.19

Flower (down-regulated transcript)

 

AY772945

Nicotiana tabacum pectin methylesterase mRNA

0.36

0.19

0.16

  1. Some clearly up- or down-regulated genes of leaf and flower samples were tested. Statistical significance was tested using Student's t-test (p < 0.05).
  2. Fold change is indicated as a ratio of HC-Pro/WT calculated from normalized median intensity values (n = 3). Standard error of mean (s.e.) is also calculated for RT-qPCR values.