Figure 4

Quantitative real-time (qPCR) assay of transcript levels of the six transcription factor genes MYBA1 , MYBA2 , MYB5A , MYB5B , MYBPA1 and MYBPA2 that regulate the flavonoid pathway in berry skin across five developmental stages of V. vinifera 'Cabernet Sauvignon' (blue dashed line) and V. aestivalis 'Norton' (red solid line). Cabernet Sauvignon berry skin were collected at 49, 59 (véraison, blue arrow), 71, 90 and 112 days after bloom (DAB), and Norton berry skin at 66, 71 (véraison, red arrow), 85, 99 and 127 DAB. Transcript abundance of each gene was normalized by the level of an actin gene. Bars indicate standard error of three biological replicates at each sampling time-point.