Figure 3
From: Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase
Subcellular localization of AtHaspin in living tobacco BY-2 cells. (A) DNA staining with Hoechst 33342 (top row), GFP fluorescence (middle row), and merged images (bottom row) showing DNA (blue) and GFP (green). Magenta arrowhead indicates fluorescent signal on chromosomes. (B) Live cell imaging was carried out in BY-2 cells expressing GFP-α-tubulin after more than 48-h induction of AtHaspin-tdTomato with 10 μM 17-β-estradiol. Merged images show AtHaspin-tdTomato (magenta) and GFP-α-tubulin (green). Magenta arrowhead indicates fluorescent signal on chromosomes. Numbers indicate time of observation (h: min) in additional file 1. (C) Kymographs representing fluorescence on yellow lines in left column. Arrows indicate PPB. Letters indicate mitotic stages as shown in (B). Scale bars: 10 μm (left), 10 min (bottom).