Figure 4
From: Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase
Phosphorylation of histone H3 at Thr3 and Thr11 in vivo. (A, B) Phosphorylation of histone H3 at Thr3 and Thr11 during cell cycle. BY-2 cells immunostained using anti-H3T3ph (A), anti-H3T11ph (B), or anti-H3S28ph antibodies. DNA was stained with DAPI. Merged images of DNA (blue), H3S10ph (red) and H3S28ph (green) are shown in color. Scale bars: 10 μm. (C) After 48-h induction with 10 μM 17-β-estradiol, BY-2 cells inducibly expressing AtHaspin-tdTomato were fixed with 4% (w/v) paraformaldehyde for 20 min. DNA was stained with DAPI. Merged images of DNA (blue) and AtHaspin-tdTomato (red) are shown in color. Scale bars: 10 μm.