Figure 5
Impact of cadmium (Cd) and/or G. irregulare inoculation (Gi) on 2-DE shoot proteins profiles of M. truncatula relative to those non-treated; 600 μg phenol-extracted proteins were separated onto first-dimensional pH 3-10 non-linear IPG strips and second dimensional 12% vertical slab gels. Mw, Bio-Rad 2-DE molecular mass standards. Representative gels from Cd-treated plants (A), G. irregulare-inoculated plants (B), G. intraradices-inoculated Cd-treated plants (C) and non-treated control plants (D). Up- and down-headed arrows represent significant (n = 3, p < 0.05) increased and decreased protein abundance reproducibly quantified in two independent biological experiments compared to non-treated control plants, respectively.