Figure 6
Comparison of the groups of protein spots that responded to G. irregulare inoculation and/or cadmium treatment in 3-w old M. truncatula shoots. Clustered abundance pattern using GENESIS software of the 23 shoot proteins of M. truncatula reproducibly detected as differentially accumulated (n = 3) in two independent biological experiments in response to one or both treatments. Log2 expression ratios were calculated for the different treatments (Cd, Gi and CdGi) either relative to the control reference (C) or to G. irregulare-inoculated plants (Gi). The green colour (-1) indicates the highest down-accumulation and the red colour (+1) the highest up-accumulation. Dark boxes (0) indicate no changes in protein abundance compared to control condition. Each row of coloured boxes is representative of a single protein and each ratio per treatment is represented using a single column, Gi/C: G intraradices-inoculated compared to control, Cd/C: Cd-treated compared to control, CdGi/C: G irregulare-inoculated and Cd-treated compared to control and CdGi/Gi: Cd treated and G. irregulare-inoculated compared to G. irregulare-inoculated plants. Asterisks indicate significant differences (p < 0.05). Protein spots are numbered according to Figure 5.